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Title: Detection of transgenes in genetically modified soybean and maize using polymerase chain reaction
Authors: Randhawa, Gurinder Jit
Firke, P K
Keywords: genetically modified soybean and maize
Issue Date: Oct-2006
Publisher: CSIR
Series/Report no.: Int. Cl.<sup>8</sup> C12N15/10
Abstract: Polymerase chain reaction (PCR) has been successfully employed to detect transgenes in genetically modified (GM) soybean and maize. Three pairs of primers for <i style="">CaMV</i> 35S, <i style="">NOS</i> and <i style="">EPSPS</i> genes were used in the detection. The results reveal that PCR could differentiate the GM plants from non-GM plants. Evidently, PCR is a highly reproducible and sensitive technique that can be successfully employed in detecting transgenes for screening GM soybeans and GM maize from their conventional plants.
Description: 510-513
ISSN: 0975-0967 (Online); 0972-5849 (Print)
Appears in Collections:IJBT Vol.05(4) [October 2006]

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