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Title: Detection of transgenes in genetically modified soybean and maize using polymerase chain reaction
Authors: Randhawa, Gurinder Jit
Firke, P K
Keywords: genetically modified soybean and maize;PCR;transgenes
Issue Date: Oct-2006
Publisher: CSIR
IPC Code: Int. Cl.8 C12N15/10
Abstract: Polymerase chain reaction (PCR) has been successfully employed to detect transgenes in genetically modified (GM) soybean and maize. Three pairs of primers for CaMV 35S, NOS and EPSPS genes were used in the detection. The results reveal that PCR could differentiate the GM plants from non-GM plants. Evidently, PCR is a highly reproducible and sensitive technique that can be successfully employed in detecting transgenes for screening GM soybeans and GM maize from their conventional plants.
Page(s): 510-513
ISSN: 0975-0967 (Online); 0972-5849 (Print)
Appears in Collections:IJBT Vol.05(4) [October 2006]

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