Please use this identifier to cite or link to this item: http://nopr.niscair.res.in/handle/123456789/5615
Title: Isolation and characterization of L-asparaginase from marine actinomycetes
Authors: Dhevagi, P
Poorani, E
Keywords: marine actinomycetes
therapeutic L-asparaginase
anti-lymphoma activity
Issue Date: Oct-2006
Publisher: CSIR
Series/Report no.: Int. Cl.<sup>8</sup> C12N9/00; C12R1/01
Abstract: <smarttagtype namespaceuri="urn:schemas-microsoft-com:office:smarttags" name="State"><smarttagtype namespaceuri="urn:schemas-microsoft-com:office:smarttags" name="place"> The marine environment is a potential source of novel actinomycetes, which are a potent source of antibiotics and novel bioactive compounds. Marine actinomycetes were enumerated in sediment samples from Parangipettai and Cochin coastal areas of South India (PDK7 and PDK2). The isolates showed potential L-asparaginase activity. The partially purified L-asparaginase showed a specific activity of 64.07 IU/mg protein, 83-fold pure and yielded 2.18 per cent of protein. The enzyme activity was maximum between <i style="">p</i>H 8 and 9 and maintained stability at <i style="">p</i>H 8. The L-asparaginase showed maximum activity at 60°C, and stable up to 80°C. The enzyme has a molecular weight of 140 kDa. It showed cytostatic effect on JURKAT cells (Acute T cell leukemia) and K562 cells (Chronic myelogenous leukemia) at 24 h and cytotoxic effects at 48 h. </smarttagtype></smarttagtype>
Description: 514-520
URI: http://hdl.handle.net/123456789/5615
ISSN: 0975-0967 (Online); 0972-5849 (Print)
Appears in Collections:IJBT Vol.05(4) [October 2006]

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