NISCAIR Online Periodicals Repository

NISCAIR ONLINE PERIODICALS REPOSITORY (NOPR)  >
NISCAIR PUBLICATIONS >
Research Journals >
Indian Journal of Biotechnology (IJBT) >
IJBT Vol.05 [2006] >
IJBT Vol.05(4) [October 2006] >


Title: Isolation and characterization of L-asparaginase from marine actinomycetes
Authors: Dhevagi, P
Poorani, E
Keywords: marine actinomycetes
therapeutic L-asparaginase
anti-lymphoma activity
Issue Date: Oct-2006
Publisher: CSIR
IPC CodeInt. Cl.8 C12N9/00; C12R1/01
Abstract:  The marine environment is a potential source of novel actinomycetes, which are a potent source of antibiotics and novel bioactive compounds. Marine actinomycetes were enumerated in sediment samples from Parangipettai and Cochin coastal areas of South India (PDK7 and PDK2). The isolates showed potential L-asparaginase activity. The partially purified L-asparaginase showed a specific activity of 64.07 IU/mg protein, 83-fold pure and yielded 2.18 per cent of protein. The enzyme activity was maximum between pH 8 and 9 and maintained stability at pH 8. The L-asparaginase showed maximum activity at 60°C, and stable up to 80°C. The enzyme has a molecular weight of 140 kDa. It showed cytostatic effect on JURKAT cells (Acute T cell leukemia) and K562 cells (Chronic myelogenous leukemia) at 24 h and cytotoxic effects at 48 h.
Page(s): 514-520
ISSN: 0975-0967 (Online); 0972-5849 (Print)
Source:IJBT Vol.05(4) [October 2006]

Files in This Item:

File Description SizeFormat
IJBT 5(4) 514-520.pdf371.66 kBAdobe PDFView/Open
 Current Page Visits: 1129 
Recommend this item

 

National Knowledge Resources Consortium |  NISCAIR Website |  Contact us |  Feedback

Disclaimer: NISCAIR assumes no responsibility for the statements and opinions advanced by contributors. The editorial staff in its work of examining papers received for publication is helped, in an honorary capacity, by many distinguished engineers and scientists.

CC License Except where otherwise noted, the Articles on this site are licensed under Creative Commons License: CC Attribution-Noncommercial-No Derivative Works 2.5 India

Copyright © 2012 The Council of Scientific and Industrial Research, New Delhi. All rights reserved.

Powered by DSpace Copyright © 2002-2007 MIT and Hewlett-Packard | Compliant to OAI-PMH V 2.0

Home Page Total Visits: 584474 since 06-Feb-2009  Last updated on 15-Sep-2014Webmaster: nopr@niscair.res.in