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Title: Ovine interferon-<img src='/image/spc_char/gamma2.gif'> gene of indigenous sheep: Cloning, sequencing and expression studies in <i style="">Escherichia coli</i>
Authors: Rasool, T J
Hosamani, M
Premraj, A
Sreekumar, E
Singh, R K
Keywords: cDNA
interferon gamma
Issue Date: Oct-2006
Publisher: CSIR
Series/Report no.: Int. Cl.<sup>8</sup> C12N15/09, 15/21
Abstract: Ovine interferon-gamma (IFN-<img src='/image/spc_char/gamma2.gif'>) cDNA was isolated from total mRNA of peripheral blood mononuclear cells from indigenous sheep by reverse transcription PCR. The cDNA was successfully cloned, sequenced and expressed in <i style="">Escherichia coli</i> as thioredoxin fusion protein. Sequence comparison of Indian sheep IFN-<img src='/image/spc_char/gamma2.gif'> showed high nucleotide homology with published IFN-<img src='/image/spc_char/gamma2.gif'> sequences of sheep and other species of ruminants. The cDNA codes for a mature polypeptide with a putative molecular weight of 17 kDa. The recombinant ovine IFN-<img src='/image/spc_char/gamma2.gif'>, expressed in BL21 <i style="">E</i>. <i style="">coli</i> cells as His-tagged protein, was purified using Ni-chelation chromatography. Up to 250 µg of recombinant ovine IFN-<img src='/image/spc_char/gamma2.gif'> could be obtained from 50 mL of bacterial culture. The production of this important cytokine has significant implications in therapy of major infectious diseases of small ruminants. It also has potential applications as adjuvant to enhance the protective efficacy of the existing vaccines.
Description: 486-490
ISSN: 0975-0967 (Online); 0972-5849 (Print)
Appears in Collections:IJBT Vol.05(4) [October 2006]

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