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Title: A simple method for extraction of high molecular weight genomic DNA from buccal cells in mouthwash
Authors: Koppikar, Priya
Mulherkar, Rita
Keywords: mouthwash samples;genomic DNA extraction;PCR
Issue Date: Oct-2006
Publisher: CSIR
IPC Code: Int. Cl.8 C12N15/10
Abstract: With tremendous advances made in the field of molecular biology techniques, the analysis of genomic DNA has become a matter of routine. However, there is a growing need for better, cost-effective, non-invasive methods for sample collection and DNA extraction. A simple protocol for sample collection and extraction of high molecular weight genomic DNA from buccal cells in mouthwash has been standardized. The method does not require elaborate instrumentation and can be applied to large population based studies. It has been observed that good quality high molecular weight genomic DNA can be obtained from exfoliated buccal cells in the early morning mouthwash samples, and that the DNA yield from similar samples decreases during the day, with very low yields obtained in the late evening. The amount of DNA obtained is enough for approximately 300 PCR amplifications using 100 ng DNA as template. DNA so obtained was used successfully for numerous amplifications by PCR with human gene specific primers and can be used for Restriction Fragment Length Polymorphism (RFLP) studies as well as for other molecular biology applications.
Page(s): 477-481
ISSN: 0975-0967 (Online); 0972-5849 (Print)
Appears in Collections:IJBT Vol.05(4) [October 2006]

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