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|Title:||Micropropagation of Capparis spinosa L. subsp. rupestris Sibth. & Sm. by nodal cuttings|
|Keywords:||caper;Capparis spinosa subsp. rupestris;tissue culture;micropropagation|
|IPC Code:||Int. Cl.8 A01H4/00|
|Abstract:||In vitro propagation of Capparis spinosa subsp. rupestris was conducted using single nodal cuttings. Multiple shoots were obtained on Murashige and Skoog (MS) medium supplemented with 1 mg/L zeatin. Shoot multiplication was optimized on the same medium by subculturing shoot segments with 2-3 nodes every 6 weeks. Aptitude to proliferation was successfully maintained during nine subcultures with a mean rate exceeding 20 new shoots per explant. High rooting response of shoots (92%) was obtained after 4 h pulse treatment period in darkness with 100 mg/L IAA solution, followed by culture on solid half-strength MS basal medium. These results indicate the enormous potential of C. spinosa subsp. rupestris that could be used for large-scale multiplication.|
|ISSN:||0975-0967 (Online); 0972-5849 (Print)|
|Appears in Collections:||IJBT Vol.05(4) [October 2006]|
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