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|Title:||Micropropagation of <i style="">Capparis spinosa </i>L. subsp. <i style="">rupestris</i> Sibth. & Sm. by nodal cuttings|
<i style="">Capparis spinosa </i>subsp.<i style=""> rupestris</i>
|Series/Report no.:||Int. Cl.<sup>8</sup> A01H4/00|
|Abstract:||<i style="">In vitro</i> propagation of <i style="">Capparis spinosa</i> subsp. <i style="">rupestris</i> was conducted using single nodal cuttings. Multiple shoots were obtained on Murashige and Skoog (MS) medium supplemented with 1 mg/L zeatin. Shoot multiplication was optimized on the same medium by subculturing shoot segments with 2-3 nodes every 6 weeks. Aptitude to proliferation was successfully maintained during nine subcultures with a mean rate exceeding 20 new shoots per explant. High rooting response of shoots (92%) was obtained after 4 h pulse treatment period in darkness with 100 mg/L IAA solution, followed by culture on solid half-strength MS basal medium. These results indicate the enormous potential of <i style="">C. spinosa</i> subsp. <i style="">rupestris</i> that could be used for large-scale multiplication.|
|ISSN:||0975-0967 (Online); 0972-5849 (Print)|
|Appears in Collections:||IJBT Vol.05(4) [October 2006]|
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