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Title: Micropropagation of Capparis spinosa L. subsp. rupestris Sibth. & Sm. by nodal cuttings
Authors: Chalak, L
Elbitar, A
Keywords: caper
Capparis spinosa subsp. rupestris
tissue culture
Issue Date: Oct-2006
Publisher: CSIR
IPC CodeInt. Cl.8 A01H4/00
Abstract: In vitro propagation of Capparis spinosa subsp. rupestris was conducted using single nodal cuttings. Multiple shoots were obtained on Murashige and Skoog (MS) medium supplemented with 1 mg/L zeatin. Shoot multiplication was optimized on the same medium by subculturing shoot segments with 2-3 nodes every 6 weeks. Aptitude to proliferation was successfully maintained during nine subcultures with a mean rate exceeding 20 new shoots per explant. High rooting response of shoots (92%) was obtained after 4 h pulse treatment period in darkness with 100 mg/L IAA solution, followed by culture on solid half-strength MS basal medium. These results indicate the enormous potential of C. spinosa subsp. rupestris that could be used for large-scale multiplication.
Page(s): 555-558
ISSN: 0975-0967 (Online); 0972-5849 (Print)
Source:IJBT Vol.05(4) [October 2006]

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