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Indian Journal of Biotechnology (IJBT) >
IJBT Vol.05 [2006] >
IJBT Vol.05(4) [October 2006] >
| Title: | Micropropagation of Capparis spinosa L. subsp. rupestris Sibth. & Sm. by nodal cuttings |
| Authors: | Chalak, L
Elbitar, A |
| Keywords: | caper
Capparis spinosa subsp. rupestris
tissue culture
micropropagation |
| Issue Date: | Oct-2006 |
| Publisher: | CSIR |
| IPC Code: | Int. Cl.8 A01H4/00 |
| Abstract: | In vitro propagation of Capparis spinosa subsp. rupestris was conducted using single nodal cuttings. Multiple shoots were obtained on Murashige and Skoog (MS) medium supplemented with 1 mg/L zeatin. Shoot multiplication was optimized on the same medium by subculturing shoot segments with 2-3 nodes every 6 weeks. Aptitude to proliferation was successfully maintained during nine subcultures with a mean rate exceeding 20 new shoots per explant. High rooting response of shoots (92%) was obtained after 4 h pulse treatment period in darkness with 100 mg/L IAA solution, followed by culture on solid half-strength MS basal medium. These results indicate the enormous potential of C. spinosa subsp. rupestris that could be used for large-scale multiplication. |
| Page(s): | 555-558 |
| ISSN: | 0975-0967 (Online); 0972-5849 (Print) |
| Source: | IJBT Vol.05(4) [October 2006]
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