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Title: Microbial degradation of melanoidins in distillery spent wash by an indigenous isolate
Authors: Chavan, M N
Kulkarni, M V
Zope, V P
Mahulikar, P P
Keywords: biodegradation;decolorisation;melanoidins;spent wash
Issue Date: Jul-2006
Publisher: CSIR
IPC Code: Int. Cl.8 CO2F3/34; CO2F101/30
Abstract: Spent wash is pollution intensive wastewater generated by distilleries. Its dark brown colour is due to recalcitrant melanoidin pigments. Bacterial cultures were screened for the ability to degrade these pigments. One isolate, identified as Pseudomonas sp., was selected for degradation studies. Spent wash dilution up to 10% and addition of externally added carbon source was essential for decolorisation. The optimum conditions for decolorisation were pH 6.8-7.2, temperature 30-35°C and glucose 0.4 g%, (W/V) of the spent wash medium. The maximum decolorisation up to 56% and 63% reduction in COD of the spent wash could be achieved after 72 h by the microbial treatment. Spectrophotometric and HPLC analysis of treated effluent confirmed biodegradation of melanoidin pigments by the isolate. This approach could be used to develop a cost-effective, eco-friendly biotechnology package for the bioremediation of spent wash before its disposal.
Page(s): 416-421
ISSN: 0975-0967 (Online); 0972-5849 (Print)
Appears in Collections:IJBT Vol.05(3) [July 2006]

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