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Indian Journal of Biotechnology (IJBT) >
IJBT Vol.05 [2006] >
IJBT Vol.05(3) [July 2006] >
| Title: | Efficient Pseudomonas putida for degradation of p-nitrophenol |
| Authors: | Kulkarni, Meenal
Chaudhari, Ambalal |
| Keywords: | p-nitrophenol
biodegradation
Pseudomonas putida
hydroquinone
ring fission |
| Issue Date: | Jul-2006 |
| Publisher: | CSIR |
| IPC Code: | Int. Cl.7 B09C1/10 |
| Abstract: | Exploration of Pseudomonas putida for controlling environmental (p-nitrophenol) PNP pollution revealed that 0.5 OD inoculum at 600 nm degraded 100 ppm of PNP completely under the ambient conditions within 24 h as established by UV-visible spectrophotometric and TLC analysis of treated broth and confirmed the same by HPLC analysis. An attempt to elucidate the mechanism of PNP degradation indicated that (i) it was oxidative with a stoichiometric release of nitrite, (ii) PNP was converted to hydroquinone, which accumulated in the presence of 2,2'-dipyridyl, (iii) hydroquinone was the first product of ring oxygenation and nitrite removal, as confirmed by TLC and HPLC analysis and (iv) concomitant formation of a keto acid by ring fission, which was confirmed by Rothera's test. |
| Page(s): | 411-415 |
| ISSN: | 0975-0967 (Online); 0972-5849 (Print) |
| Source: | IJBT Vol.05(3) [July 2006]
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