Please use this identifier to cite or link to this item: http://nopr.niscair.res.in/handle/123456789/5599
Title: Selection of optimal growth medium for the synthesis of <img src='/image/spc_char/alpha.gif'> -galactosidase from mangrove actinomycetes
Authors: Anisha, G S
Prema, P
Keywords: <img src='/image/spc_char/alpha.gif'>-galactosidase
mangrove actinomycetes
locust bean gum
crude enzyme
characterization
Issue Date: Jul-2006
Publisher: CSIR
Series/Report no.: Int. Cl.<sup>8</sup> C12N9/40; C12R1/01
Abstract: <img src='/image/spc_char/alpha.gif'> -Galactosidases (<img src='/image/spc_char/alpha.gif'> -galactoside galactohydrolase, EC.3.2.1.22) are exoglycosidases that cleave terminal <img src='/image/spc_char/alpha.gif'> -1→6-linked galactose residues from glycoconjugates. To select best medium for the enhanced production of this enzyme by the mangrove actinomycete cultures (AGP 42 and AGP 47), four different media reported in literature were tried. The medium containing locust bean gum as the carbon source (Medium I) gave the highest level of <img src='/image/spc_char/alpha.gif'> -galactosidase activity and was selected for further studies. The actinomycete culture AGP 42 was found to give higher enzyme titers than AGP 47. A comparison of the characteristics of crude <img src='/image/spc_char/alpha.gif'> -galactosidase from these cultures showed that the enzyme from AGP 42 was active over a wider range of <i style="">p</i>H (4.0 to 8.5) compared to that from AGP 47 (<i style="">p</i>H 5.5 to 8.5). The optimum <i style="">p</i>H for the maximum <img src='/image/spc_char/alpha.gif'> -galactosidase activity from AGP 42 and AGP 47 was 7.0 and 6.4, respectively. Similarly, the optimum temperatures for both the cultures were 55 and 40°C. The enzyme from AGP 42 was found to be stable at <i style="">p</i>H range 6.0 to 7.5 and that from AGP 47 at <i style="">p</i>H range 6.0 to 7.0. Similarly, the temperature stability of <img src='/image/spc_char/alpha.gif'> -galactosidase from both the cultures was from 30 to 45°C and 30 to 40°C.
Description: 373-379
URI: http://hdl.handle.net/123456789/5599
ISSN: 0975-0967 (Online); 0972-5849 (Print)
Appears in Collections:IJBT Vol.05(3) [July 2006]

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