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Title: Entrapment of lipase in polymer of polyvinyl alcohol-boric acid for esterification in organic media
Authors: Dave, Rachna
Madamwar, Datta
Keywords: boric acid;entrapment;ethyl butyrate;lipase;polyvinyl alcohol
Issue Date: Jul-2006
Publisher: CSIR
IPC Code: Int. Cl.8 C12N9/20; C12N11/08; C12R1/72
Abstract: Polyvinyl alcohol (PVA)-boric acid method has been utilized for entrapment of Candida rugosa lipase. Immobilized lipase was used to produce ethyl butyrate, a flavour ester showing 80.2% conversion in 72 h. Lipase in PVA-boric acid beads possessed the ability to synthesize variety of esters and was stable in various organic solvents with varying log P value from 2 to 8 under incubation at 50°C for 1 h.The immobilized lipase showed nearly full retention of activity even after 8 cycles of use, the activity then gradually decreased reaching to 56% conversion efficiency after 20 cycles and possesses a shelf-life of 10 months. The thermostability of the lipase increased three times upon immobilization. The immobilized enzyme possessed 40% higher activity compared to its free counterpart.
Page(s): 368-372
ISSN: 0975-0967 (Online); 0972-5849 (Print)
Appears in Collections:IJBT Vol.05(3) [July 2006]

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