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Title: Characterization of chitinases from microorganisms isolated from Lonar lake
Authors: Bansode, Vijay B
Bajekal, Shyam S
Keywords: alkaline
fungal protoplasting
seafood waste
Issue Date: Jul-2006
Publisher: CSIR
IPC CodeInt. Cl.8 A01N63/04
Abstract: Chitinases, the enzymes that breakdown chitin—the second most abundant polysaccharide in nature—are known to have numerous uses. Alkaline chitinases, in particular are considered to have a greater potential in this respect. Fifteen chitinolytic microbial strains were isolated from the alkaline soil of Lonar lake in Buldhana district of Maharashtra. In this paper, the characters of chitinases produced by the five most potent isolates are presented. All the enzymes exhibited maximum activity in the neutral to alkaline range of pH (from 7.0 to 9.6) with a wide stability range from pH 4 to 11. The temperature optima for all the enzymes were slightly on the higher side, between 35 and 60°C, with a stability range from 25 to 60°C. All the enzymes showed a typical response to substrate concentration. None of the enzymes had any specific requirement for any particular metal ion, though a considerable stimulatory effect of Ca2+ was observed on all the enzymes. Minor effects of Cu2+, Mn2+ and Na1+ were also observed on some of the enzymes.
Page(s): 357-363
ISSN: 0975-0967 (Online); 0972-5849 (Print)
Source:IJBT Vol.05(3) [July 2006]

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