NISCAIR Online Periodicals Repository

Research Journals >
Indian Journal of Biotechnology (IJBT) >
IJBT Vol.05 [2006] >
IJBT Vol.05(3) [July 2006] >

Title: Purification and characterization of an endoxylanase from solid-state culture of alkalitolerant Aspergillus fumigatus MKU1
Authors: Thiagarajan, S
Jeya, M
Gunasekaran, P
Keywords: Aspergillus fumigatus
biochemical characterization
solid state culture
Issue Date: Jul-2006
Publisher: CSIR
IPC CodeInt. Cl.8 C12N9/42; C12R1/68
Abstract: A novel high molecular weight endoxylanase (XylF1) from the solid state culture of Aspergillus fumigatus MKU1 strain was purified to homogeneity by a combination of tube gel electrophoresis and electroelution methods. The purity of XylF1 was demonstrated by SDS-PAGE, which has the molecular mass of 43 kDa. The optimal pH and temperature for enzyme activity were 7.0 and 70° C, respectively. Further, the apparent Km and Vmax values of XylF1 with oat spelt xylan as substrate were 6.25 mg/mL and 0.05 mmol/mL/min (2.5 mmol/min/mg protein), respectively. The enzyme showed high activity on oat spelt xylan, while only trace of activity was observed on carboxy methylcellulose. The activity of XylF1 was strongly inhibited by Hg 2+, Cu 2+, Fe3+ and NBS, whereas stimulated by DTT, DTNB and L-Cysteine. The hydrolysis of oat spelt xylan released only xylooligosaccharides.
Page(s): 351-356
ISSN: 0975-0967 (Online); 0972-5849 (Print)
Source:IJBT Vol.05(3) [July 2006]

Files in This Item:

File Description SizeFormat
IJBT 5(3) 351-356.pdf286.3 kBAdobe PDFView/Open
 Current Page Visits: 1169 
Recommend this item


Online Submission of Articles |  NISCAIR Website |  National Knowledge Resources Consortium |  Contact us |  Feedback

Disclaimer: NISCAIR assumes no responsibility for the statements and opinions advanced by contributors. The editorial staff in its work of examining papers received for publication is helped, in an honorary capacity, by many distinguished engineers and scientists.

CC License Except where otherwise noted, the Articles on this site are licensed under Creative Commons License: CC Attribution-Noncommercial-No Derivative Works 2.5 India

Copyright © 2015 The Council of Scientific and Industrial Research, New Delhi. All rights reserved.

Powered by DSpace Copyright © 2002-2007 MIT and Hewlett-Packard | Compliant to OAI-PMH V 2.0

Home Page Total Visits: 167517 since 01-Sep-2015  Last updated on 28-Jun-2016Webmaster: