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Title: Production of a high maltose-forming, hyperthermostable and Ca<sup>2+</sup>- independent amylopullulanase by an extreme thermophile <i style="">Geobacillus thermoleovorans</i> in submerged fermentation
Authors: Noorwez, S M
Ezhilvannan, M
Satyanarayana, T
Keywords: <i style="">Geobacillus thermoleovorans</i>
submerged fermentation
starch hydrolysis
Issue Date: Jul-2006
Publisher: CSIR
Series/Report no.: Int. Cl.<sup>8</sup> C12N9/44; C12R1/01
Abstract: Production of a hyperthermostable, high maltose-forming and Ca<sup>2+</sup>-independent amylopullulanase by an extreme thermophile <i style="">Geobacillus thermoleovorans</i> was studied in submerged fermentation. Parametric optimization led to secretion of amylopullulanase (2850 U L<sup>-1</sup> of <img src='/image/spc_char/alpha.gif'> -amylase, 840 U L<sup>-1</sup> of pullulanase) in a starch-yeast extract medium (2% starch, 0.1% ammonium sulphate, 0.3% yeast extract, 0.1% K<sub>2</sub>HPO<sub>4</sub>, 0.1% NaCl, 0.01% MgSO<sub>4</sub>.7H<sub>2</sub>O and 0.1% Maltose) using 2% inoculum (12 h) at 70°C and 200 rpm in 20 h. The addition of trace elements, detergents, surfactants and additives did not stimulate growth or enzyme production. The hydrolysis of pullulan and starch yielded maltotriose, and maltose, maltotriose and maltotetraose as the major end products, respectively. The cultivation of the organism in a laboratory fermenter showed (i) abolition of the lag phase, (ii) a 2-fold increase in enzyme production (5880 U L<sup>-1</sup> of <img src='/image/spc_char/alpha.gif'> -amylase, 1900 U L<sup>-1</sup> of pullulanase) and (iii) a reduction in fermentation time to 7-8 h.
Description: 337-345
ISSN: 0975-0967 (Online); 0972-5849 (Print)
Appears in Collections:IJBT Vol.05(3) [July 2006]

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