NISCAIR Online Periodicals Repository

NISCAIR ONLINE PERIODICALS REPOSITORY (NOPR)  >
NISCAIR PUBLICATIONS >
Research Journals >
Indian Journal of Biotechnology (IJBT) >
IJBT Vol.05 [2006] >
IJBT Vol.05(3) [July 2006] >


Title: Production of a high maltose-forming, hyperthermostable and Ca2+- independent amylopullulanase by an extreme thermophile Geobacillus thermoleovorans in submerged fermentation
Authors: Noorwez, S M
Ezhilvannan, M
Satyanarayana, T
Keywords: Geobacillus thermoleovorans
amylopullulanase
submerged fermentation
starch hydrolysis
Issue Date: Jul-2006
Publisher: CSIR
IPC CodeInt. Cl.8 C12N9/44; C12R1/01
Abstract: Production of a hyperthermostable, high maltose-forming and Ca2+-independent amylopullulanase by an extreme thermophile Geobacillus thermoleovorans was studied in submerged fermentation. Parametric optimization led to secretion of amylopullulanase (2850 U L-1 of -amylase, 840 U L-1 of pullulanase) in a starch-yeast extract medium (2% starch, 0.1% ammonium sulphate, 0.3% yeast extract, 0.1% K2HPO4, 0.1% NaCl, 0.01% MgSO4.7H2O and 0.1% Maltose) using 2% inoculum (12 h) at 70°C and 200 rpm in 20 h. The addition of trace elements, detergents, surfactants and additives did not stimulate growth or enzyme production. The hydrolysis of pullulan and starch yielded maltotriose, and maltose, maltotriose and maltotetraose as the major end products, respectively. The cultivation of the organism in a laboratory fermenter showed (i) abolition of the lag phase, (ii) a 2-fold increase in enzyme production (5880 U L-1 of -amylase, 1900 U L-1 of pullulanase) and (iii) a reduction in fermentation time to 7-8 h.
Page(s): 337-345
ISSN: 0975-0967 (Online); 0972-5849 (Print)
Source:IJBT Vol.05(3) [July 2006]

Files in This Item:

File Description SizeFormat
IJBT 5(3) 337-345.pdf411.6 kBAdobe PDFView/Open
 Current Page Visits: 661 
Recommend this item

 

National Knowledge Resources Consortium |  NISCAIR Website |  Contact us |  Feedback

Disclaimer: NISCAIR assumes no responsibility for the statements and opinions advanced by contributors. The editorial staff in its work of examining papers received for publication is helped, in an honorary capacity, by many distinguished engineers and scientists.

CC License Except where otherwise noted, the Articles on this site are licensed under Creative Commons License: CC Attribution-Noncommercial-No Derivative Works 2.5 India

Copyright © 2012 The Council of Scientific and Industrial Research, New Delhi. All rights reserved.

Powered by DSpace Copyright © 2002-2007 MIT and Hewlett-Packard | Compliant to OAI-PMH V 2.0

Home Page Total Visits: 607242 since 06-Feb-2009  Last updated on 31-Oct-2014Webmaster: nopr@niscair.res.in