Please use this identifier to cite or link to this item:
Title: Production of a high maltose-forming, hyperthermostable and Ca2+- independent amylopullulanase by an extreme thermophile Geobacillus thermoleovorans in submerged fermentation
Authors: Noorwez, S M
Ezhilvannan, M
Satyanarayana, T
Keywords: Geobacillus thermoleovorans;amylopullulanase;submerged fermentation;starch hydrolysis
Issue Date: Jul-2006
Publisher: CSIR
IPC Code: Int. Cl.8 C12N9/44; C12R1/01
Abstract: Production of a hyperthermostable, high maltose-forming and Ca2+-independent amylopullulanase by an extreme thermophile Geobacillus thermoleovorans was studied in submerged fermentation. Parametric optimization led to secretion of amylopullulanase (2850 U L-1 of -amylase, 840 U L-1 of pullulanase) in a starch-yeast extract medium (2% starch, 0.1% ammonium sulphate, 0.3% yeast extract, 0.1% K2HPO4, 0.1% NaCl, 0.01% MgSO4.7H2O and 0.1% Maltose) using 2% inoculum (12 h) at 70°C and 200 rpm in 20 h. The addition of trace elements, detergents, surfactants and additives did not stimulate growth or enzyme production. The hydrolysis of pullulan and starch yielded maltotriose, and maltose, maltotriose and maltotetraose as the major end products, respectively. The cultivation of the organism in a laboratory fermenter showed (i) abolition of the lag phase, (ii) a 2-fold increase in enzyme production (5880 U L-1 of -amylase, 1900 U L-1 of pullulanase) and (iii) a reduction in fermentation time to 7-8 h.
Page(s): 337-345
ISSN: 0975-0967 (Online); 0972-5849 (Print)
Appears in Collections:IJBT Vol.05(3) [July 2006]

Files in This Item:
File Description SizeFormat 
IJBT 5(3) 337-345.pdf411.6 kBAdobe PDFView/Open

Items in NOPR are protected by copyright, with all rights reserved, unless otherwise indicated.