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IJBT Vol.05 [2006] >
IJBT Vol.05(3) [July 2006] >
| Title: | Solid-state fermentation of lignocellulosic substrates for cellulase production by Trichoderma reesei NRRL 11460 |
| Authors: | Singhania, Reeta Rani
Sukumaran, Rajeev K
Pillai, Anu
Prema, P
Szakacs, George
Pandey, Ashok |
| Keywords: | cellulase
endoglucanse
Trichoderma
solid-state fermentation
lignocellulosic residues
sugarcane bagasse |
| Issue Date: | Jul-2006 |
| Publisher: | CSIR |
| IPC Code: | Int. Cl.8 C12N9/42; C12R1/885 |
| Abstract: | Cellulase production studies were carried out using the fungal culture Trichoderma reesei NRRL 11460 using four different lignocellulosic residues (both raw and pre-treated) by solid-state fermentation. The effect of basic fermentation parameters on enzyme production was studied. Maximal cellulase production obtained was 154.58 U/gds when pre-treated sugarcane bagasse (PSCB) was used as substrate. The optimal conditions for cellulase production using PSCB were found to be initial moisture content - 66%, initial medium pH-7.0, incubation temperature -28°C, NH4NO3 at 0.075 M, and 0.005 M cellobiose. The optimal incubation time for production was 72 h. Results indicate the scope for further optimization of the production conditions to obtain higher cellulase titres using the strain under SSF. |
| Page(s): | 332-336 |
| ISSN: | 0975-0967 (Online); 0972-5849 (Print) |
| Source: | IJBT Vol.05(3) [July 2006]
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