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Indian Journal of Biotechnology (IJBT) >
IJBT Vol.05 [2006] >
IJBT Vol.05(3) [July 2006] >
| Title: | Isolation and characterization of recombinant Brugian parasitic transglutaminase |
| Authors: | Uma, V
Geetha, M
Murugan, V
Kaliraj, P |
| Keywords: | adjuvants
filaricidals
immunoprophylaxis
molting
nematodes
protein disulphide isomerase
transgluta-minase
vaccine |
| Issue Date: | Jul-2006 |
| Publisher: | CSIR |
| IPC Code: | Int. Cl.8 C12N9/64 |
| Abstract: | An attempt has been made to identify and characterize a putative chemotherapeutic target antigen for human lymphatic filariasis by recombinant DNA techniques. Identification of transglutaminase (TGA), an enzyme vital for the growth and survival of the parasites, has opened a new drug target for developing potent therapeutic agents against the parasites. TGA gene from Brugia malayi adult female parasite (BmTGA) has been subcloned, expressed and purified as a 47 kDa fusion protein with N-terminal histidine tag. Since BmTGA lacks homology to mammalian TGAs, it is a promising drug target for developing chemotherapeutic agent and candidate vaccine against human lymphatic filariasis. |
| Page(s): | 317-326 |
| ISSN: | 0975-0967 (Online); 0972-5849 (Print) |
| Source: | IJBT Vol.05(3) [July 2006]
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