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Title:  Genetic variability in pea wilt pathogen Fusarium oxysporum f. sp. pisi in north-western Himalayas
Authors: Sharma, Pooja
Sharma, Kamal Dev
Sharma, Rajan
Plaha, P
Keywords: cultural characteristics;Fusarium oxysporum f. sp. pisi;protein markers;RAPD;variability
Issue Date: Jul-2006
Publisher: CSIR
IPC Code: Int. Cl.8 C12N15/10
Abstract:  Genetic diversity in Fusarium oxysporum f. sp. pisi isolates from three agroclimatically distinct regions, sub-tropical, sub-humid and wet-temperate, of north-western Himalayas was studied using cultural characteristics, DNA (RAPD) and protein (native-proteins and esterase isozyme) markers. Variability in growth pattern, colour of mycelium and pigments was observed. Based on cultural characters, 24 isolates from three regions could be assigned to six groups. Amplification of genomic DNA of 24 isolates with ten 10-mer primers generated 134 polymorphic markers, whereas native-protein and esterase profiles revealed 27 markers. Based on NTSYS analysis of RAPD and protein data, the isolates were delineated into four region specific groups. Group PRI, PRIII and PRIV represented isolates from sub-tropical and sub-humid regions, whereas group PRII consisted of isolates from dry-temperate region, indicating that pathogen populations from sub tropical and sub humid regions evolved from three distinct lineages and those from temperate regions from the fourth lineage.
Page(s): 298-302
ISSN: 0975-0967 (Online); 0972-5849 (Print)
Appears in Collections:IJBT Vol.05(3) [July 2006]

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