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Title: Properties of hydrogel-entrapped lipase of thermophilic Pseudomonas aeruginosa BTS-2
Authors: Kanwar, S S
Gupta, M
Gupta, R
Kaushal, R K
Chimni, S S
Keywords: esterification
Pseudomonas aeruginosa BTS-2
stability in alcohols/alkanes
Issue Date: Jul-2006
Publisher: CSIR
IPC CodeInt. Cl.8 C12N9/20; C12N11/04; C12R1/385
Abstract: Pseudomonas aeruginosa BTS-2 produced lipase (total yield, 55.4 U/L) optimally on a mineral based (MB) broth, supplemented with 1% (v/v) cottonseed oil as sole carbon source, after incubation at 56°C for 48 h. The presence of 4% (w/v) yeast extract in the MB broth further increased the lipase production by ~ 3-folds. The purified lipase was efficiently (~80%) immobilized in alginate than carrageenan beads. The alginate-immobilized lipase (beads) showed optima at pH 7.5 and 55°C temperature. An increase in C-chain of alcohols increased the activity of entraped lipase. Exposure of alginate beads to decyl (C:10) and dodecyl (C:12) alcohol enhanced the lipase activity by 47 and 64%, respectively. Whereas, most alkanes decreased the activity of immobilized lipase but exposure to nonane enhanced the activity by 52%. The lipase efficiently esterified ethanol and acetic acid to ethyl acetate.
Page(s): 292-297
ISSN: 0975-0967 (Online); 0972-5849 (Print)
Source:IJBT Vol.05(3) [July 2006]

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