NISCAIR Online Periodicals Repository

NISCAIR ONLINE PERIODICALS REPOSITORY (NOPR)  >
NISCAIR PUBLICATIONS >
Research Journals >
Indian Journal of Biotechnology (IJBT) >
IJBT Vol.05 [2006] >
IJBT Vol.05(3) [July 2006] >


Title: Properties of hydrogel-entrapped lipase of thermophilic Pseudomonas aeruginosa BTS-2
Authors: Kanwar, S S
Gupta, M
Gupta, R
Kaushal, R K
Chimni, S S
Keywords: esterification
immobilization
Pseudomonas aeruginosa BTS-2
stability in alcohols/alkanes
Issue Date: Jul-2006
Publisher: CSIR
IPC CodeInt. Cl.8 C12N9/20; C12N11/04; C12R1/385
Abstract: Pseudomonas aeruginosa BTS-2 produced lipase (total yield, 55.4 U/L) optimally on a mineral based (MB) broth, supplemented with 1% (v/v) cottonseed oil as sole carbon source, after incubation at 56°C for 48 h. The presence of 4% (w/v) yeast extract in the MB broth further increased the lipase production by ~ 3-folds. The purified lipase was efficiently (~80%) immobilized in alginate than carrageenan beads. The alginate-immobilized lipase (beads) showed optima at pH 7.5 and 55°C temperature. An increase in C-chain of alcohols increased the activity of entraped lipase. Exposure of alginate beads to decyl (C:10) and dodecyl (C:12) alcohol enhanced the lipase activity by 47 and 64%, respectively. Whereas, most alkanes decreased the activity of immobilized lipase but exposure to nonane enhanced the activity by 52%. The lipase efficiently esterified ethanol and acetic acid to ethyl acetate.
Page(s): 292-297
ISSN: 0975-0967 (Online); 0972-5849 (Print)
Source:IJBT Vol.05(3) [July 2006]

Files in This Item:

File Description SizeFormat
IJBT 5(3) 292-297.pdf57.57 kBAdobe PDFView/Open
 Current Page Visits: 664 
Recommend this item

 

National Knowledge Resources Consortium |  NISCAIR Website |  Contact us |  Feedback

Disclaimer: NISCAIR assumes no responsibility for the statements and opinions advanced by contributors. The editorial staff in its work of examining papers received for publication is helped, in an honorary capacity, by many distinguished engineers and scientists.

CC License Except where otherwise noted, the Articles on this site are licensed under Creative Commons License: CC Attribution-Noncommercial-No Derivative Works 2.5 India

Copyright © 2012 The Council of Scientific and Industrial Research, New Delhi. All rights reserved.

Powered by DSpace Copyright © 2002-2007 MIT and Hewlett-Packard | Compliant to OAI-PMH V 2.0

Home Page Total Visits: 638127 since 06-Feb-2009  Last updated on 15-Dec-2014Webmaster: nopr@niscair.res.in