Please use this identifier to cite or link to this item: http://nopr.niscair.res.in/handle/123456789/5583
Title: Development of DNA probe for detection of egg drop syndrome-76 virus
Authors: Kavitha, K L
Rao, V D P
Suryanarayana, V V S
Keywords: Egg drop syndrome-76
Indian isolate
DNA probe
radiolabelling
specificity
sensitivity
Issue Date: Apr-2006
Publisher: CSIR
Series/Report no.: Int. Cl.<sup>8</sup> C12N15/10
Abstract: <smarttagtype namespaceuri="urn:schemas-microsoft-com:office:smarttags" name="State"><smarttagtype namespaceuri="urn:schemas-microsoft-com:office:smarttags" name="City"><smarttagtype namespaceuri="urn:schemas-microsoft-com:office:smarttags" name="place"> Two kb, <i style="">Bam</i>HI fragment of duck embryo propagated Indian isolate (VN 1) of egg drop syndrome-76 (EDS-76) was cloned into pBluescript II KS + vector and the fragment was labelled with <img src='/image/spc_char/alpha.gif'> <sup>33</sup>p dATP label using random primer extension method. The labelled DNA was used as a probe for detecting viral DNA in faecal samples of experimentally infected birds. Proteinase K treated, phenol extracted, alkali denatured samples exhibited strong signals as compared to proteinase K digested samples alone. The probe could detect up to 1.25 pg of viral DNA. However, it did not show any positive signal with another adenovirus, i.e., hydropericardium syndrome viral DNA indicating its specificity for egg drop syndrome virus (EDSV). </smarttagtype></smarttagtype></smarttagtype>
Description: 229-231
URI: http://hdl.handle.net/123456789/5583
ISSN: 0975-0967 (Online); 0972-5849 (Print)
Appears in Collections:IJBT Vol.05(2) [April 2006]

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