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Indian Journal of Biotechnology (IJBT) >
IJBT Vol.05 [2006] >
IJBT Vol.05(2) [April 2006] >
| Title: | Development of DNA probe for detection of egg drop syndrome-76 virus |
| Authors: | Kavitha, K L
Rao, V D P
Suryanarayana, V V S |
| Keywords: | Egg drop syndrome-76
Indian isolate
DNA probe
radiolabelling
specificity
sensitivity |
| Issue Date: | Apr-2006 |
| Publisher: | CSIR |
| IPC Code: | Int. Cl.8 C12N15/10 |
| Abstract: | Two kb, BamHI fragment of duck embryo propagated Indian isolate (VN 1) of egg drop syndrome-76 (EDS-76) was cloned into pBluescript II KS + vector and the fragment was labelled with  33p dATP label using random primer extension method. The labelled DNA was used as a probe for detecting viral DNA in faecal samples of experimentally infected birds. Proteinase K treated, phenol extracted, alkali denatured samples exhibited strong signals as compared to proteinase K digested samples alone. The probe could detect up to 1.25 pg of viral DNA. However, it did not show any positive signal with another adenovirus, i.e., hydropericardium syndrome viral DNA indicating its specificity for egg drop syndrome virus (EDSV). |
| Page(s): | 229-231 |
| ISSN: | 0975-0967 (Online); 0972-5849 (Print) |
| Source: | IJBT Vol.05(2) [April 2006]
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