NISCAIR Online Periodicals Repository

NISCAIR ONLINE PERIODICALS REPOSITORY (NOPR)  >
NISCAIR PUBLICATIONS >
Research Journals >
Indian Journal of Biotechnology (IJBT) >
IJBT Vol.05 [2006] >
IJBT Vol.05(2) [April 2006] >


Title: Development of DNA probe for detection of egg drop syndrome-76 virus
Authors: Kavitha, K L
Rao, V D P
Suryanarayana, V V S
Keywords: Egg drop syndrome-76
Indian isolate
DNA probe
radiolabelling
specificity
sensitivity
Issue Date: Apr-2006
Publisher: CSIR
IPC CodeInt. Cl.8 C12N15/10
Abstract:  Two kb, BamHI fragment of duck embryo propagated Indian isolate (VN 1) of egg drop syndrome-76 (EDS-76) was cloned into pBluescript II KS + vector and the fragment was labelled with 33p dATP label using random primer extension method. The labelled DNA was used as a probe for detecting viral DNA in faecal samples of experimentally infected birds. Proteinase K treated, phenol extracted, alkali denatured samples exhibited strong signals as compared to proteinase K digested samples alone. The probe could detect up to 1.25 pg of viral DNA. However, it did not show any positive signal with another adenovirus, i.e., hydropericardium syndrome viral DNA indicating its specificity for egg drop syndrome virus (EDSV).
Page(s): 229-231
ISSN: 0975-0967 (Online); 0972-5849 (Print)
Source:IJBT Vol.05(2) [April 2006]

Files in This Item:

File Description SizeFormat
IJBT 5(2) 229-231.pdf413.8 kBAdobe PDFView/Open
 Current Page Visits: 495 
Recommend this item

 

National Knowledge Resources Consortium |  NISCAIR Website |  Contact us |  Feedback

Disclaimer: NISCAIR assumes no responsibility for the statements and opinions advanced by contributors. The editorial staff in its work of examining papers received for publication is helped, in an honorary capacity, by many distinguished engineers and scientists.

CC License Except where otherwise noted, the Articles on this site are licensed under Creative Commons License: CC Attribution-Noncommercial-No Derivative Works 2.5 India

Copyright © 2012 The Council of Scientific and Industrial Research, New Delhi. All rights reserved.

Powered by DSpace Copyright © 2002-2007 MIT and Hewlett-Packard | Compliant to OAI-PMH V 2.0

Home Page Total Visits: 559904 since 06-Feb-2009  Last updated on 30-Jul-2014Webmaster: nopr@niscair.res.in