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Title:  Cytochrome oxidase I sequence of Helicoverpa (Noctuidae: Lepidoptera) species in India—Its utility as a molecular tool
Authors: Kranthi, S
Kranthi, K R
Bharose, A A
Syed, S N
Dhawad, C S
Wadaskar, R M
Behere, G T
Patil, E K
Keywords: CO-I sequence;Drosophila yakuba;Helicoverpa assulta;H. armigera
Issue Date: Apr-2006
Publisher: CSIR
IPC Code: Int. Cl.8 C12N15/10, 15/52
Abstract:  The genus Helicoverpa comprises of 2 species in India—H. armigera and H. assulta. This paper compares partial CO-1 sequences of the two-field-collected species with a laboratory strain of H. armigera and Drosophila yakuba whose entire mitochondrial genome has been sequenced. The region sequenced in the study corresponds to 2111 to 2601 bp sequence of D. yakuba mitochondrial genome, i.e mid to near terminal segment of the CO-I region. When analyzed, at least 18 nucleotide and 8 amino acid substitutions were observed between the two species. Using this information, a specific PCR-RFLP tool was designed that distinguishes between the two species at the egg stage itself, thus, influencing pest control options significantly, especially in areas and crops where the two may occur simultaneously.
Page(s): 195-199
ISSN: 0975-0967 (Online); 0972-5849 (Print)
Appears in Collections:IJBT Vol.05(2) [April 2006]

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