Please use this identifier to cite or link to this item: http://nopr.niscair.res.in/handle/123456789/5578
Title: <img src='/image/spc_char/beta.gif'>-Galactosidase from <i style="">Lactobacillus acidophilus </i>isolated from fermented ragi (<i style="">Eleusine coracana</i>)
Authors: Akolkar, S K
Sajgure, A D
Lele, S S
Keywords: <i style="">Lactobacillus acidophilus</i>
<i style="">Eleusine coracana</i>
<img src='/image/spc_char/beta.gif'>-galactosidase
Issue Date: Apr-2006
Publisher: CSIR
Series/Report no.: Int. Cl.<sup>8</sup> C12N 19/38; C12R1:23
Abstract: <i style="">Lactobacillus acidophilus</i> was isolated from fermented millet, <i style="">Eleusine coracana</i>. It was characterized using several biochemical tests. This strain was found to be homofermentative, slime forming and <img src='/image/spc_char/beta.gif'>-galactosidase producer. Recovery and characterization of <img src='/image/spc_char/beta.gif'>-galactosidase were studied at laboratory scale. Since the enzyme was intracellular various cell lysis methods were studied to achieve maximum enzyme release from cell fragments. Homogenization at a pressure of 2000 psig two passes showed an enzyme release of 2050 U/mL and was found the best method for cell lysis. The cell extract was purified using ultrafiltration and gel permeation chromatography. Specific activity and fold purification of <img src='/image/spc_char/beta.gif'>-galactosidase was found to be 568.61 and 21.2, respectively. Kinetic parameters were determined using ONPG (o-nitrophenyl galactopyranoside) as a substrate. Michaeli’s Menten equation was found to fit the reaction of ONPG using <img src='/image/spc_char/beta.gif'>-galactosidase from <i style="">L. acidophilus. </i>Vmax and Km were calculated from the Lineweaver Burk plot. Vmax was found to be 4.94 min<sup>-1</sup> and Km to be 0.11 m<i style="">M</i>. Using gel permeation chromatography, the molecular weight was found to be in the range of 450-500 kDa.
Description: 184-188
URI: http://hdl.handle.net/123456789/5578
ISSN: 0975-0967 (Online); 0972-5849 (Print)
Appears in Collections:IJBT Vol.05(2) [April 2006]

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