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|Title:||Purification and immobilization of an <i style="">Aspergillus terreus</i> xylanase: Use of continuous fluidized bed column reactor|
|Series/Report no.:||Int. Cl.<sup>8</sup> A01N63/02, C12N9/24, C12N11/04|
|Abstract:||An <i style="">Aspergillus terreus</i> extracellular xylanase produced by solid state fermentation was purified and characterized. A 6.4 fold purified xylanase was obtained by ammonium sulphate (in 30-40% saturation) precipitation followed by dialysis. Molecular weight of the xylanase was 67.0 kDa as estimated by SDS-PAGE. The enzyme was immobilized in barium alginate gel. Both free and immobilized enzyme showed maximum activity at <i style="">p</i>H 5.5 and 60°C (8.0 IU/mg & 5.25 IU/mg, respectively) and were most stable at <i style="">p</i>H 5.5 and thermostable up to 55°C. Co<sup>2+</sup> stimulated free enzyme activity (9.27 IU/mg) and Mg<sup>2+</sup> stimulated activity of immobilized enzyme (5.54 IU/mg). V<sub>max</sub> and K<sub>m</sub> for free and immobilized xylanases were 6.6 <img src='/image/spc_char/micro.gif'> mole/mg/min, 0.75% and 1.25 <img src='/image/spc_char/micro.gif'> mole/mg/min, 0.625%, respectively. 23.4% conversion of substrate (0.1% birchwood xylan) was possible in 7 h using a continuous fluidized bed column reactor under the following conditions: bed height 16 cm, temperature 40°C, and dilution rate 4.09 h<sup>-1</sup>.|
|ISSN:||0975-0967 (Online); 0972-5849 (Print)|
|Appears in Collections:||IJBT Vol.05(2) [April 2006]|
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