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Title: | Purification and immobilization of an Aspergillus terreus xylanase: Use of continuous fluidized bed column reactor |
Authors: | Pal, Anju Ray, Lalitagauri Chattopadhyay, Parimal |
Keywords: | xylanase;purification;immobilization;continuous conversion |
Issue Date: | Apr-2006 |
Publisher: | CSIR |
IPC Code: | Int. Cl.8 A01N63/02, C12N9/24, C12N11/04 |
Abstract: | An Aspergillus terreus extracellular xylanase produced by solid state fermentation was purified and characterized. A 6.4 fold purified xylanase was obtained by ammonium sulphate (in 30-40% saturation) precipitation followed by dialysis. Molecular weight of the xylanase was 67.0 kDa as estimated by SDS-PAGE. The enzyme was immobilized in barium alginate gel. Both free and immobilized enzyme showed maximum activity at pH 5.5 and 60°C (8.0 IU/mg & 5.25 IU/mg, respectively) and were most stable at pH 5.5 and thermostable up to 55°C. Co2+ stimulated free enzyme activity (9.27 IU/mg) and Mg2+ stimulated activity of immobilized enzyme (5.54 IU/mg). Vmax and Km for free and immobilized xylanases were 6.6 ![]() ![]() |
Page(s): | 163-168 |
URI: | http://hdl.handle.net/123456789/5574 |
ISSN: | 0975-0967 (Online); 0972-5849 (Print) |
Appears in Collections: | IJBT Vol.05(2) [April 2006] |
Files in This Item:
File | Description | Size | Format | |
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IJBT 5(2) 163-168.pdf | 218.99 kB | Adobe PDF | View/Open |
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