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|Title:||Polygalacturonases: Active site analyses and mechanism of action|
|Keywords:||polygalacturonases;protein sequence analysis;active site amino acids;distance conservation between motifs;mechanism of action|
|IPC Code:||Int. Cl.8 C12N9/38, 9/40|
|Abstract:||Polygalacturonases from various sources have been analyzed by ClustalW and T-COFFEE, for identification of conserved and functional motifs in them. All the 104 polygalacturonases analyzed by the above programs, revealed four highly conserved motifs, viz., NTD, G/QDD, G/SHG and RIK among them. Distance conservation between the motifs was also observed. Based on the available evidences from chemical modification studies on active site amino acids, site-directed mutagenesis, protein sequence analysis and X-ray crystallographic data, a mechanism of action is proposed for this group of enzymes.|
|ISSN:||0975-0967 (Online); 0972-5849 (Print)|
|Appears in Collections:||IJBT Vol.05(2) [April 2006]|
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