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Indian Journal of Biotechnology (IJBT) >
IJBT Vol.05 [2006] >
IJBT Vol.05(2) [April 2006] >
| Title: | Polygalacturonases: Active site analyses and mechanism of action |
| Authors: | Palanivelu, P |
| Keywords: | polygalacturonases
protein sequence analysis
active site amino acids
distance conservation between motifs
mechanism of action |
| Issue Date: | Apr-2006 |
| Publisher: | CSIR |
| IPC Code: | Int. Cl.8 C12N9/38, 9/40 |
| Abstract: | Polygalacturonases from various sources have been analyzed by ClustalW and T-COFFEE, for identification of conserved and functional motifs in them. All the 104 polygalacturonases analyzed by the above programs, revealed four highly conserved motifs, viz., NTD, G/QDD, G/SHG and RIK among them. Distance conservation between the motifs was also observed. Based on the available evidences from chemical modification studies on active site amino acids, site-directed mutagenesis, protein sequence analysis and X-ray crystallographic data, a mechanism of action is proposed for this group of enzymes. |
| Page(s): | 148-162 |
| ISSN: | 0975-0967 (Online); 0972-5849 (Print) |
| Source: | IJBT Vol.05(2) [April 2006]
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