Please use this identifier to cite or link to this item:
Title: Polygalacturonases: Active site analyses and mechanism of action
Authors: Palanivelu, P
Keywords: polygalacturonases
protein sequence analysis
active site amino acids
distance conservation between motifs
mechanism of action
Issue Date: Apr-2006
Publisher: CSIR
Series/Report no.: Int. Cl.8 C12N9/38, 9/40
Abstract: Polygalacturonases from various sources have been analyzed by ClustalW and T-COFFEE, for identification of conserved and functional motifs in them. All the 104 polygalacturonases analyzed by the above programs, revealed four highly conserved motifs, viz., NTD, G/QDD, G/SHG and RIK among them. Distance conservation between the motifs was also observed. Based on the available evidences from chemical modification studies on active site amino acids, site-directed mutagenesis, protein sequence analysis and X-ray crystallographic data, a mechanism of action is proposed for this group of enzymes.
Description: 148-162
ISSN: 0975-0967 (Online); 0972-5849 (Print)
Appears in Collections:IJBT Vol.05(2) [April 2006]

Files in This Item:
File Description SizeFormat 
IJBT 5(2) 148-162.pdf3.25 MBAdobe PDFView/Open

Items in NOPR are protected by copyright, with all rights reserved, unless otherwise indicated.