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Title: Immobilization of Pleurotus ostreatus 1804 on PUF cubes: Influence of mycelial growth pattern on laccase yield
Authors: Prasad, K Krishna
Mohan, S Venkata
Pati, B R
Sarma, P N
Keywords: laccase
immobilization
PUF cubes
mycelial aggregation
SEM imaging
SDS-PAGE electrophoresis
Issue Date: Jan-2006
Publisher: CSIR
IPC CodeInt. Cl.7 A01N63/04; C12N9/00, 11/08; C12R1:645
Abstract: The influence of immobilized Pleurotus ostreatus 1804, on polyurethane foam (PUF) cubes, was investigated on laccase yield as a function of immobilized PUF cubes and their repeated application. Enhanced laccase expression after fermentation was observed with immobilized PUF cubes (3109 U in 168 h) compared to free mycelia (2583 U in 192 h). The expression of laccase during fermentation was monitored by SDS-PAGE electrophoresis, which showed a band with a molecular mass of 63 kDa. Effective laccase yield was also observed with repeated application of immobilized PUF cubes consistently upto 8 batches of fermentation. Scanning electron microscope (SEM) images revealed the pattern of spiraling colonization and aggregation of the P. ostreatus mycelia on PUF cubes. During repeated application, the hyphal growth on the PUF cubes increased consistently, manifested by both the processes of tip extension and branching, permitting the fungal colonization.
Page(s): 84-88
ISSN: 0975-0967 (Online); 0972-5849 (Print)
Source:IJBT Vol.05(1) [January 2006]

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