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Title: Optimization of process parameters for alkaline protease production under solid-state fermentation by <i style="">Thermoactinomyces thalpophilus </i>PEE 14
Authors: Divakar, G
Sunitha, M
Vasu, P
shanker, P Udaya
Ellaiah, P
Keywords: alkaline protease
<i style="">Thermoactinmyces</i> <i style="">thalpophilus </i>PEE 14
wheat bran
Issue Date: Jan-2006
Publisher: CSIR
Series/Report no.: Int. Cl.<sup>7</sup> C12N9/50; C12R1:01
Abstract: Production parameters of extracellular alkaline protease employing our laboratory isolate <i style="">Thermoactinomyces thalpophilus </i>PEE 14<i style=""> </i>under solid-state fermentation (SSF) were optimized. Wheat bran the best substrate among the 15 substrates used for optimization, showed the highest activity (1620 PU/g). The physical and chemical parameters were also optimized. The maximum enzyme activity under optimum conditions was obtained with incubation period 72 h, incubation temperature 55ºC, initial <i style="">p</i>H 10, inoculum level 20%, level of salt solution 2:10 and initial moisture level 80%. Increase of enzyme activity by 60% (2576 PU/g) was observed when compared with the unoptimized conditions.
Description: 80-83
ISSN: 0975-0967 (Online); 0972-5849 (Print)
Appears in Collections:IJBT Vol.05(1) [January 2006]

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