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Title: Amplification and cloning of canine parvovirus VP1 gene into mammalian expression vector
Authors: Gupta, P K
Raut, A A
Rai, A
Keywords: Canine parvovirus;capsid protein;VP1 gene;PCR;cloning;plasmid vector
Issue Date: Jan-2006
Publisher: CSIR
IPC Code: Int. Cl.7 C12N15/09
Abstract: The VP1 gene of canine parvovirus was amplified by polymerase chain reaction using the thermostable polymerase with proof reading activity. Taq DNA polymerase was used to add ‘A’ overhang in the PCR product. This ‘A’ tailed PCR product was cloned in TA cloning vector pTargeT and transformed in Escherichia coli JM109 cells. The recombinant plasmid containing the VP1 gene insert was screened and characterized using restriction enzyme analysis and nested PCR.
Page(s): 117-119
ISSN: 0975-0967 (Online); 0972-5849 (Print)
Appears in Collections:IJBT Vol.05(1) [January 2006]

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