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IJBT Vol.05(1) [January 2006] >


Title: RAPD analysis of genetic variability in Pinus gerardiana Wall. in Kinnaur (Himachal Pradesh)
Authors: Kant, Anil
Pattanayak, D
Chakrabarti, S K
Sharma, Rajan
Thakur, Manisha
Sharma, D R
Keywords: Chilgoza pine
genetic diversity
Himalayas
Pinus gerardiana
RAPD
Issue Date: Jan-2006
Publisher: CSIR
IPC CodeInt. Cl.7 C12N15/10
Abstract: RAPD analysis was carried out to determine the genetic diversity of Pinus gerardiana in Himachal Pradesh. Twenty-four genotypes of P. gerardiana growing at different locations in the distribution range of species were sampled randomly for analysis. Thirty random primers generated 413 fragments, of which 390 (94%) were polymorphic in nature. Eleven of the 24 individual genotypes were distinguished by unique bands specific to them. The similarity coefficient values suggested a wide genetic base of genotypes taken in the experiment. An UPGMA dendrogram based on similarity coefficient indices indicated that genotypes did not cluster according to their site of collection. This can be attributed to highly cross-pollinating nature of the species and small distributional range in the area.
Page(s): 62-67
ISSN: 0975-0967 (Online); 0972-5849 (Print)
Source:IJBT Vol.05(1) [January 2006]

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