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IJBT Vol.06(4) [October 2007] >


Title: Plant regeneration in different genotypes of indica rice
Authors: Biswas, Anusrita
Mandal, Asit B
Keywords: Genotypes
rice
media
adjuvant
hormones
Issue Date: Oct-2007
Publisher: CSIR
IPC CodeInt. Cl.8 A01H4/00
Abstract: Of 10 indica rice varieties assessed, Annada showed the best in vitro culture response in respect of callus induction, plantlet regeneration and number of plantlets/seed callus. Other high yielding varieties, viz. Kasturi, IR 72 and Karnal Local, also displayed appreciable performance. Callus induced and proliferated in dark and gave high plantlet regeneration and more plantlets/seed callus across genotypes. Among three shock treatments, viz. heat (37°C for overnight), cold (4°C for over night) and dehydration, dehydration significantly enhanced the plantlet regeneration and number of plantlets/seed callus. Of 3-dehydration treatments (6, 12 and 24 h duration), the maximum plantlet regeneration and number of plantlets/seed callus were observed in the calli dehydrated for 12 h. Of three tested media, viz. MS, LS, and N6, MS emerged the best for callus induction, plantlet regeneration and number of plantlets/seed callus. In contrast, among the modified media formulations, modified N6 [5 mM (NH4)2 SO4] produced maximum plantlets/seed callus across varieties. Increased (5 mM) (NH4)2SO4 content in the medium was found to be better than the higher dose of KNO3 or its normal level in the respective media formulations. Across genotypes, 2 mg L-1 2,4-D in the callus induction medium (CIM) was the most suitable for callus induction. However, 2,4-D in combination with other growth regulators, such as 2 mg L-1 2,4-D and 1 mg L-1 Kn, performed better. In regeneration medium (RM), 2 mg L-1 BAP with 1 mg L-1 Kn and 0.5 mg L-1 NAA, or 2 mg L-1 BAP with 0.5 mg L-1 NAA, were optimum for the maximum plantlet regeneration. Across varieties, coconut water (10%) was found to be the most effective to enhance the in vitro culture response as evident from prolific callus induction, increased plantlet regeneration and more plantlets/seed callus.
Page(s): 532-540
ISSN: 0975-0967 (Online); 0972-5849 (Print)
Source:IJBT Vol.06(4) [October 2007]

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