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Title: Biolistic-mediated DNA delivery and transient expression of GUS in hypocotyls of <i style="">Feronia limonia</i> L.—A fruit tree
Authors: Purohit, S D
Raghuvanshi, S
Tyagi, A K
Keywords: biolistic
<i style="">Feronia limonia</i>
transient expression
Issue Date: Oct-2007
Publisher: CSIR
Series/Report no.: Int. Cl.<sup>8</sup> A01H15/00; C12N15/09, 15/11
Abstract: <i style="">Feronia limonia</i> L. (Rutaceae) is an important fruit tree of arid horticulture. To be able to introduce genes of interest for its improvement, biolistic-mediated DNA delivery method has been standardized. The hypocotyls explants were bombarded with plasmid pBI121 having <i style="">gus</i> reporter gene driven by CaMV 35S promoter and <i style="">npt</i> II under control of <i style="">nos</i> promoter as selectable marker. The best transient expression of GUS was recorded when tungsten particles coated with DNA were bombarded at a disc pressure of 1350 and 1100 psi from a distance of 9 cm. Some of the bombarded explants are being maintained to obtain viable regenerates for their analyses.
Description: 504-507
ISSN: 0975-0967 (Online); 0972-5849 (Print)
Appears in Collections:IJBT Vol.06(4) [October 2007]

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