Please use this identifier to cite or link to this item:
Title: Biolistic-mediated DNA delivery and transient expression of GUS in hypocotyls of Feronia limonia L.—A fruit tree
Authors: Purohit, S D
Raghuvanshi, S
Tyagi, A K
Keywords: biolistic;Feronia limonia;transient expression
Issue Date: Oct-2007
Publisher: CSIR
IPC Code: Int. Cl.8 A01H15/00; C12N15/09, 15/11
Abstract: Feronia limonia L. (Rutaceae) is an important fruit tree of arid horticulture. To be able to introduce genes of interest for its improvement, biolistic-mediated DNA delivery method has been standardized. The hypocotyls explants were bombarded with plasmid pBI121 having gus reporter gene driven by CaMV 35S promoter and npt II under control of nos promoter as selectable marker. The best transient expression of GUS was recorded when tungsten particles coated with DNA were bombarded at a disc pressure of 1350 and 1100 psi from a distance of 9 cm. Some of the bombarded explants are being maintained to obtain viable regenerates for their analyses.
Page(s): 504-507
ISSN: 0975-0967 (Online); 0972-5849 (Print)
Appears in Collections:IJBT Vol.06(4) [October 2007]

Files in This Item:
File Description SizeFormat 
IJBT 6(4) 504-507.pdf234.96 kBAdobe PDFView/Open

Items in NOPR are protected by copyright, with all rights reserved, unless otherwise indicated.