NISCAIR Online Periodicals Repository

Research Journals >
Indian Journal of Biotechnology (IJBT) >
IJBT Vol.06 [2007] >
IJBT Vol.06(4) [October 2007] >

Title: Biolistic-mediated DNA delivery and transient expression of GUS in hypocotyls of Feronia limonia L.—A fruit tree
Authors: Purohit, S D
Raghuvanshi, S
Tyagi, A K
Keywords: biolistic
Feronia limonia
transient expression
Issue Date: Oct-2007
Publisher: CSIR
IPC CodeInt. Cl.8 A01H15/00; C12N15/09, 15/11
Abstract: Feronia limonia L. (Rutaceae) is an important fruit tree of arid horticulture. To be able to introduce genes of interest for its improvement, biolistic-mediated DNA delivery method has been standardized. The hypocotyls explants were bombarded with plasmid pBI121 having gus reporter gene driven by CaMV 35S promoter and npt II under control of nos promoter as selectable marker. The best transient expression of GUS was recorded when tungsten particles coated with DNA were bombarded at a disc pressure of 1350 and 1100 psi from a distance of 9 cm. Some of the bombarded explants are being maintained to obtain viable regenerates for their analyses.
Page(s): 504-507
ISSN: 0975-0967 (Online); 0972-5849 (Print)
Source:IJBT Vol.06(4) [October 2007]

Files in This Item:

File Description SizeFormat
IJBT 6(4) 504-507.pdf234.96 kBAdobe PDFView/Open
 Current Page Visits: 1187 
Recommend this item


Online Submission of Articles |  NISCAIR Website |  National Knowledge Resources Consortium |  Contact us |  Feedback

Disclaimer: NISCAIR assumes no responsibility for the statements and opinions advanced by contributors. The editorial staff in its work of examining papers received for publication is helped, in an honorary capacity, by many distinguished engineers and scientists.

CC License Except where otherwise noted, the Articles on this site are licensed under Creative Commons License: CC Attribution-Noncommercial-No Derivative Works 2.5 India

Copyright © 2015 The Council of Scientific and Industrial Research, New Delhi. All rights reserved.

Powered by DSpace Copyright © 2002-2007 MIT and Hewlett-Packard | Compliant to OAI-PMH V 2.0

Home Page Total Visits: 166333 since 01-Sep-2015  Last updated on 28-Jun-2016Webmaster: