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Indian Journal of Biotechnology (IJBT) >
IJBT Vol.06 [2007] >
IJBT Vol.06(4) [October 2007] >
| Title: | Isolation of pigeon pea (Cajanus cajan L.) legumin gene promoter and identification of conserved regulatory elements using tools of bioinformatics |
| Authors: | Jaiswal, Rajani
Nain, Vikrant
Abdin, M Z
Kumar, P A |
| Keywords: | β-glucuronidase (GUS)
legumin gene
nucleosome positioning
phylogenetic footprinting |
| Issue Date: | Oct-2007 |
| Publisher: | CSIR |
| IPC Code: | Int. Cl.8 C12N15/09, 15/29 |
| Abstract: | A seed specific legumin gene promoter from pigeon pea was isolated by PCR amplification. Database assisted sequence analysis of this promoter revealed several putative cis-acting regulatory elements. Comparative analysis of 15 seed-specific legumin gene promoters from six species, viz. Cajanus cajan, Cicer arietinum, Pisum sativum, Glycine max, Vicia faba and Arachis hypogaea, revealed several conserved motifs in promoter sequences; maximum conservation was observed upstream to transcription start site. Most of the conserved motifs have known transcription factor binding sites. One unknown conserved motif of seven base pair (AG/TGTGTA) was found 19 bp upstream to legumin box, putatively named as L-19. Study of nucleosome formation potential showed that putative linker DNA is more prone to mutations as compared to DNA involved in nucleosome formation. A chimeric construct was made with pigeonpea legumin promoter and β-glucuronidase (GUS) gene. Analysis of GUS expression at different developmental stages of transgenic tobacco plant’s parts revealed that the reporter gene was expressed at a high level only in mature seeds, specifically in embryo, endosperm and in cotyledonary leaves of developing seedling. These data showed that GUS gene transcription was regulated in a tissue specific and temporally regulated manner. |
| Page(s): | 495-503 |
| ISSN: | 0975-0967 (Online); 0972-5849 (Print) |
| Source: | IJBT Vol.06(4) [October 2007]
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