NISCAIR Online Periodicals Repository

Research Journals >
Indian Journal of Biotechnology (IJBT) >
IJBT Vol.06 [2007] >
IJBT Vol.06(4) [October 2007] >

Title: Isolation of pigeon pea (Cajanus cajan L.) legumin gene promoter and identification of conserved regulatory elements using tools of bioinformatics
Authors: Jaiswal, Rajani
Nain, Vikrant
Abdin, M Z
Kumar, P A
Keywords: β-glucuronidase (GUS)
legumin gene
nucleosome positioning
phylogenetic footprinting
Issue Date: Oct-2007
Publisher: CSIR
IPC CodeInt. Cl.8 C12N15/09, 15/29
Abstract: A seed specific legumin gene promoter from pigeon pea was isolated by PCR amplification. Database assisted sequence analysis of this promoter revealed several putative cis-acting regulatory elements. Comparative analysis of 15 seed-specific legumin gene promoters from six species, viz. Cajanus cajan, Cicer arietinum, Pisum sativum, Glycine max, Vicia faba and Arachis hypogaea, revealed several conserved motifs in promoter sequences; maximum conservation was observed upstream to transcription start site. Most of the conserved motifs have known transcription factor binding sites. One unknown conserved motif of seven base pair (AG/TGTGTA) was found 19 bp upstream to legumin box, putatively named as L-19. Study of nucleosome formation potential showed that putative linker DNA is more prone to mutations as compared to DNA involved in nucleosome formation. A chimeric construct was made with pigeonpea legumin promoter and β-glucuronidase (GUS) gene. Analysis of GUS expression at different developmental stages of transgenic tobacco plant’s parts revealed that the reporter gene was expressed at a high level only in mature seeds, specifically in embryo, endosperm and in cotyledonary leaves of developing seedling. These data showed that GUS gene transcription was regulated in a tissue specific and temporally regulated manner.
Page(s): 495-503
ISSN: 0975-0967 (Online); 0972-5849 (Print)
Source:IJBT Vol.06(4) [October 2007]

Files in This Item:

File Description SizeFormat
IJBT 6(4) 495-503.pdf1.22 MBAdobe PDFView/Open
 Current Page Visits: 1319 
Recommend this item


Online Submission of Articles |  NISCAIR Website |  National Knowledge Resources Consortium |  Contact us |  Feedback

Disclaimer: NISCAIR assumes no responsibility for the statements and opinions advanced by contributors. The editorial staff in its work of examining papers received for publication is helped, in an honorary capacity, by many distinguished engineers and scientists.

CC License Except where otherwise noted, the Articles on this site are licensed under Creative Commons License: CC Attribution-Noncommercial-No Derivative Works 2.5 India

Copyright © 2015 The Council of Scientific and Industrial Research, New Delhi. All rights reserved.

Powered by DSpace Copyright © 2002-2007 MIT and Hewlett-Packard | Compliant to OAI-PMH V 2.0

Home Page Total Visits: 163726 since 01-Sep-2015  Last updated on 21-Jun-2016Webmaster: