Please use this identifier to cite or link to this item:
|Title:||Antileishmanial apoptotic activity of Nigella sativa L. essential oil and thymoquinone triggers on Leishmania tropica|
|Authors:||Al-Turkmani, Mohamad Osama|
|Keywords:||Black Cumin;Cell cycle arrest;Cutaneous leishmaniasis;Programmed cell death;Promastigotes|
|Abstract:||Nigella sativa L., commonly called Black cumin, is well-known in folk medicine and numerous studies have shown its various pharmacological activities. In this study, we estimated the cytotoxic effects of N. sativa essential oil (NEO) and its major bioactive component Thymoquinone (TQ) on Leishmania tropica promastigotes that cause cutaneous leishmanias, and also observed the programmed cell death features. The extraction of NEO was done by hydro-distillation and analyzed by Gas Chromatography-Mass Spectrometry (GC-MS). Antileishmanial activity of NEO and TQ was determined using 3-(4.5-dimethylthiazol-2-yl)-2.5-diphenyl tetrazolium bromide (MTT) assay and the obtained results are expressed as 50% inhibitory concentration (IC50). The leishmanicidal activity of NEO and TQ was mediated via apoptosis as evidenced by in situ labelling of DNA fragments using terminal deoxyribonucleotidyltransferase-mediated dUTP nick end labelling (TUNEL) and cell cycle arrest at sub G0/G1 phase. The IC50 values were 5 µg/mL and 1.3 µg/mL, respectively. We noted a significant increase in DNA fragmentation in treated parasites with IC50 of both NEO and TQ as well as a cell cycle arrest. These results revealed that NEO and TQ possess potential antileishmanial activity that mediated high possibility by programmed cell death.|
|ISSN:||0975-1009 (Online); 0019-5189 (Print)|
|Appears in Collections:||IJEB Vol.58(10) [October 2020]|
Items in NOPR are protected by copyright, with all rights reserved, unless otherwise indicated.