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dc.contributor.authorAnbu, Periasamy-
dc.contributor.authorGopinath, Subash CB-
dc.contributor.authorSo, Jae-Seong-
dc.contributor.authorJayanthi, S-
dc.identifier.issn0975-0959 (Online); 0301-1208 (Print)-
dc.description.abstractThis study has been performed to isolate a potential strain able to release the prolific amylase under non-aqueous conditions to meet the current demand in industries to substitute the amylase produced in aqueous media. A bacterial strain that produces organic solvent-stable amylase in the media containing 15% benzene was isolated from the soil. The recovered strain was identified to be Bacillus amyloliquefaciens AMY02by 16S rRNA sequencing. Under sub-merged fermentation, the optimized amylase release by this strain was found with the condition having starch (carbon source), pH 7.0, the temperature at 30°C for 48 h (incubation time). This optimized condition promoted the amylase production to be 2.04-fold higher than the culture was kept under standard condition with the basic media composition. Further, the stability of the enzyme in the presence of 20% organic solvents was assessed by incubating for 2 weeks. The enzyme was found to be active and stable in the presence of benzene, chloroform, o-xylene, and toluene. The higher organic solvent stability of this amylase production by B. amyloliquefaciens under sub-merged fermentation can be an alternative catalyst in non-aqueous media for industrial applications.en_US
dc.publisherNISCAIR-CSIR, Indiaen_US
dc.rights CC Attribution-Noncommercial-No Derivative Works 2.5 Indiaen_US
dc.sourceIJBB Vol.57(5) [October 2020]en_US
dc.subjectAmylase productionen_US
dc.subjectNon-aqueous mediaen_US
dc.subjectPhylogenetic analysisen_US
dc.subjectSolvent-stable enzymesen_US
dc.subjectSolvent tolerant bacteriaen_US
dc.titleStable release of enhanced organic solvent tolerant amylase from Bacillus amyloliquefaciens AMY02 under sub-merged fermentationen_US
Appears in Collections:IJBB Vol.57(5) [October 2020]

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