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Title: Effects of verteporfin-mediated photodynamic therapy in breast cancer cells
Authors: Sönmez, Pınar Kılıçaslan
Turhan, Ali
Öztatlıcı, Mustafa
Özbilgin, Kemal
Keywords: Bax;Bcl-2;Laser;MDA-MB-231;Photosensitizer
Issue Date: Oct-2020
Publisher: NISCAIR-CSIR, India
Abstract: Photodynamic therapy works with a photosensitizer that is stimulated when exposed to a light source of a specific wavelength and produces a form of oxygen that can be used in cancer treatments. In this study, we investigated the effect of laser on apoptosis on breast cancer cell lines (MDA-MB-231) treated with verteporfin in cell culture media. Verteporfin added MDA-MB-231 cells were incubated without light for 24 hours after applying laser light at a wavelength of 695 nm at an intensity of 50 J/cm2 at various times. Anti-proliferative effects were evaluated by immunoreactivity of anti-Bcl-2 and anti-Bax antibodies by immunocytochemical staining. When anti-Bax/Anti-Bcl-2 ratio are compared, the ratio of 1.5 in the control group cells decreases in short-term laser applications, while it approaches normal values in the 7th min after long-term laser application and reaches a very high value in the 9th min. Therefore, our results suggest that verteporfin-mediated PDT may be a potential combined therapy strategy against breast carcinoma by increasing apoptosis.
Page(s): 560-566
ISSN: 0975-0959 (Online); 0301-1208 (Print)
Appears in Collections:IJBB Vol.57(5) [October 2020]

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