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Title: In vitro propagation of Dendrobium microbulbon A. Rich―A rare ethnomedicinal herb
Authors: Sharma, Urvashi
Rao, V Rama
Mohan, J S S
Reddy, A S
Keywords: conservation;in vitro flowering;secondary explants;shoot multiplication;pseudobulbs;Dendrobium microbulbon
Issue Date: Jul-2007
Publisher: CSIR
IPC Code: Int. Cl.8 A01H4/00, 5/04
Abstract:  Dendrobium microbulbon A. Rich (Orchidaceae) is a small epiphytic rare orchid used in stomachache by the tribal people of Gujarat. The present study deals with the development of a suitable protocol for its clonal propagation using pseudobulbs as explants. Murashige and Skoog (MS) basal medium supplemented with various concentrations of auxin and cytokinin was used either alone or in combination for induction, multiplication, elongation and rooting of in vitro shoots. MS medium fortified with 3% sucrose, 7.5 mg L-1 indole-3-acetic acid (IAA) and 20 mg L-1 benzyl aminopurine (BAP) showed axillary bud initiation. MS medium with 2.0 mg L-1 BAP favoured production of maximum number of shoots (39 shoots), elongation of shoots as well as bulblets formation. In vitro raised shoots were rooted on Knop salts with MS vitamins and MS iron salts. During acclimatization, 60% of the plantlets survived after their removal from in vitro conditions.
Page(s): 381-384
ISSN: 0975-0967 (Online); 0972-5849 (Print)
Appears in Collections: IJBT Vol.06(3) [July 2007]

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