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Indian Journal of Biotechnology (IJBT) >
IJBT Vol.06 [2007] >
IJBT Vol.06(3) [July 2007] >
| Title: | In vitro propagation of Dendrobium microbulbon A. Rich―A rare ethnomedicinal herb |
| Authors: | Sharma, Urvashi
Rao, V Rama
Mohan, J S S
Reddy, A S |
| Keywords: | conservation
in vitro flowering
secondary explants
shoot multiplication
pseudobulbs
Dendrobium microbulbon |
| Issue Date: | Jul-2007 |
| Publisher: | CSIR |
| IPC Code: | Int. Cl.8 A01H4/00, 5/04 |
| Abstract: | Dendrobium microbulbon A. Rich (Orchidaceae) is a small epiphytic rare orchid used in stomachache by the tribal people of Gujarat. The present study deals with the development of a suitable protocol for its clonal propagation using pseudobulbs as explants. Murashige and Skoog (MS) basal medium supplemented with various concentrations of auxin and cytokinin was used either alone or in combination for induction, multiplication, elongation and rooting of in vitro shoots. MS medium fortified with 3% sucrose, 7.5 mg L-1 indole-3-acetic acid (IAA) and 20 mg L-1 benzyl aminopurine (BAP) showed axillary bud initiation. MS medium with 2.0 mg L-1 BAP favoured production of maximum number of shoots (39 shoots), elongation of shoots as well as bulblets formation. In vitro raised shoots were rooted on Knop salts with MS vitamins and MS iron salts. During acclimatization, 60% of the plantlets survived after their removal from in vitro conditions. |
| Page(s): | 381-384 |
| ISSN: | 0975-0967 (Online); 0972-5849 (Print) |
| Source: | IJBT Vol.06(3) [July 2007]
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