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Title: An alternative source for regenerable organogenic callus induction in Jatropha curcas L.
Authors: Rajore, Shilpa
Batra, Amla
Keywords: plant regeneration;organogenesis;Jatropha curcas; agro-forestry
Issue Date: Oct-2007
Publisher: CSIR
IPC Code: Int. Cl.8 A01H4/00
Abstract: Plant regeneration of Jatropha curcas was achieved through organogenesis in callus cultures. Calli were induced from leaf explants on MS basal medium supplemented with NAA (1.0 mg/L) and BAP (5.0 mg/L). Green compact nodules containing clusters of meristematic centres were induced in these calli after transfer to MS basal medium containing various concentrations of BAP/Kn (0.5-5.0 mg/L) alone or in combination with auxins. MS medium supplemented with BAP (1.5 mg/L) and IBA (0.5 mg/L) was found to be the most effective combination for shoot bud differentiation. The microshoots rooted well on MS+IBA (3.0 mg/L) medium and the plantlets successfully acclimatized in soil.
Page(s): 545-548
ISSN: 0975-0967 (Online); 0972-5849 (Print)
Appears in Collections:IJBT Vol.06(4) [October 2007]

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