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Research Journals >
Indian Journal of Biotechnology (IJBT) >
IJBT Vol.06 [2007] >
IJBT Vol.06(4) [October 2007] >
| Title: | An alternative source for regenerable organogenic callus induction in Jatropha curcas L. |
| Authors: | Rajore, Shilpa
Batra, Amla |
| Keywords: | plant regeneration
organogenesis
Jatropha curcas
agro-forestry |
| Issue Date: | Oct-2007 |
| Publisher: | CSIR |
| IPC Code: | Int. Cl.8 A01H4/00 |
| Abstract: | Plant regeneration of Jatropha curcas was achieved through organogenesis in callus cultures. Calli were induced from leaf explants on MS basal medium supplemented with NAA (1.0 mg/L) and BAP (5.0 mg/L). Green compact nodules containing clusters of meristematic centres were induced in these calli after transfer to MS basal medium containing various concentrations of BAP/Kn (0.5-5.0 mg/L) alone or in combination with auxins. MS medium supplemented with BAP (1.5 mg/L) and IBA (0.5 mg/L) was found to be the most effective combination for shoot bud differentiation. The microshoots rooted well on MS+IBA (3.0 mg/L) medium and the plantlets successfully acclimatized in soil. |
| Page(s): | 545-548 |
| ISSN: | 0975-0967 (Online); 0972-5849 (Print) |
| Source: | IJBT Vol.06(4) [October 2007]
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