|
NISCAIR ONLINE PERIODICALS REPOSITORY (NOPR) >
NISCAIR PUBLICATIONS >
Research Journals >
Indian Journal of Biotechnology (IJBT) >
IJBT Vol.06 [2007] >
IJBT Vol.06(4) [October 2007] >
| Title: | In vitro propagation of an endemic umbellifer, Hydrocotyle conferta |
| Authors: | Karuppusamy, S
Aruna, V
Kiranmai, C
Pullaiah, T |
| Keywords: | axillary bud multiplication
Hydrocotyle conferta
endemic umbellifer |
| Issue Date: | Oct-2007 |
| Publisher: | CSIR |
| IPC Code: | Int. Cl.8 A01H4/00 |
| Abstract: | A protocol for in vitro propagation of Hydrocotyle conferta Wight (Apiaceae) through axillary bud multiplication was established. Murashige and Skoog (MS) medium with 6.66  M N6-benzyladenine (BA) and 5.37 M  -naphthalenacetic acid (NAA) was best suited for axillary bud multiplication including a mean of 21 shoots/node. Excision and culture of the nodal segments from the in vitro shoots on fresh medium with same concentrations of BA and NAA facilitated development of more than 25 shoots/node. Subsequent cultures enhanced the rate of shoot proliferation. The developed shoots rooted best on half strength MS medium with 0.54 M NAA. Plantlets established in pots exhibited 95% survival. |
| Page(s): | 541-544 |
| ISSN: | 0975-0967 (Online); 0972-5849 (Print) |
| Source: | IJBT Vol.06(4) [October 2007]
|
|
