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Title: In vitro propagation of an endemic umbellifer, Hydrocotyle conferta
Authors: Karuppusamy, S
Aruna, V
Kiranmai, C
Pullaiah, T
Keywords: axillary bud multiplication
Hydrocotyle conferta
endemic umbellifer
Issue Date: Oct-2007
Publisher: CSIR
IPC CodeInt. Cl.8 A01H4/00
Abstract: A protocol for in vitro propagation of Hydrocotyle conferta Wight (Apiaceae) through axillary bud multiplication was established. Murashige and Skoog (MS) medium with 6.66 M N6-benzyladenine (BA) and 5.37 M -naphthalenacetic acid (NAA) was best suited for axillary bud multiplication including a mean of 21 shoots/node. Excision and culture of the nodal segments from the in vitro shoots on fresh medium with same concentrations of BA and NAA facilitated development of more than 25 shoots/node. Subsequent cultures enhanced the rate of shoot proliferation. The developed shoots rooted best on half strength MS medium with 0.54 M NAA. Plantlets established in pots exhibited 95% survival.
Page(s): 541-544
ISSN: 0975-0967 (Online); 0972-5849 (Print)
Source:IJBT Vol.06(4) [October 2007]

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