NISCAIR Online Periodicals Repository

Research Journals >
Indian Journal of Biotechnology (IJBT) >
IJBT Vol.06 [2007] >
IJBT Vol.06(3) [July 2007] >

Title: Efficient plant regeneration from cotyledons of black gram [Vigna mungo (L.) Hepper]
Authors: Varalaxmi, Y
Vijayalakshmi, A
Yadav, S K
Venkateswarlu, B
Maheswari, M
Keywords: black gram
grain legumes
in vitro culture
plant growth regulators
plant regeneration
Vigna mungo
Issue Date: Jul-2007
Publisher: CSIR
IPC CodeInt. Cl.8 A01H4/00, 5/10
Abstract: An efficient and rapid regeneration protocol was developed from cotyledons of black gram [Vigna mungo (L.) Hepper] variety T-9. Murashige and Skoog’s (MS) medium supplemented with Gamborg B5 vitamins containing BAP @ 4.0 mg/L was most effective in producing regenerative calli. The cultures expressed maximum plant regeneration potential with 12 shoots per calli on regeneration media with no exogenous amino acid or plant growth regulator supplementation. Green and robust shoots thus developed were successfully rooted within 15 d on 1/3 MS medium. Over 90% of rooted plants grew well and produced seeds normally when transferred to green house.
Page(s): 414-417
ISSN: 0975-0967 (Online); 0972-5849 (Print)
Source: IJBT Vol.06(3) [July 2007]

Files in This Item:

File Description SizeFormat
IJBT 6(3) 414-417.pdf299.38 kBAdobe PDFView/Open
 Current Page Visits: 853 
Recommend this item


Online Submission of Articles |  NISCAIR Website |  National Knowledge Resources Consortium |  Contact us |  Feedback

Disclaimer: NISCAIR assumes no responsibility for the statements and opinions advanced by contributors. The editorial staff in its work of examining papers received for publication is helped, in an honorary capacity, by many distinguished engineers and scientists.

CC License Except where otherwise noted, the Articles on this site are licensed under Creative Commons License: CC Attribution-Noncommercial-No Derivative Works 2.5 India

Copyright © 2015 The Council of Scientific and Industrial Research, New Delhi. All rights reserved.

Powered by DSpace Copyright © 2002-2007 MIT and Hewlett-Packard | Compliant to OAI-PMH V 2.0

Home Page Total Visits: 169922 since 01-Sep-2015  Last updated on 30-Jun-2016Webmaster: