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Title: Development of a dual-gene PCR assay for simultaneous amplification of structural and non-structural genes of bovine rotavirus A
Authors: Gupta, Vandana
Nayak, Anju
Swamy, Madhu
Singh, R. V.
Keywords: Bovine rotavirus;Dual-gene PCR;Structural gene;Non-structural gene diagnosis
Issue Date: Jan-2020
Publisher: NISCAIR-CSIR, India
Abstract: Rotavirus can be detected in stool samples by using serological and molecular methods. An easy and rapid dual gene RT-PCR assay for the simultaneous amplification of the capsid protein VP4 (structural) and rotavirus enter toxin NSP4 (non-structural protein) has been developed. We validated the assay by comparing the results of single target PCR with dual-gene PCR products.
Page(s): 45-48
ISSN: 0975-0967 (Online); 0972-5849 (Print)
Appears in Collections:IJBT Vol.19(1) [January 2020]

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