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|Title:||Development of a dual-gene PCR assay for simultaneous amplification of structural and non-structural genes of bovine rotavirus A|
Singh, R. V.
|Keywords:||Bovine rotavirus;Dual-gene PCR;Structural gene;Non-structural gene diagnosis|
|Abstract:||Rotavirus can be detected in stool samples by using serological and molecular methods. An easy and rapid dual gene RT-PCR assay for the simultaneous amplification of the capsid protein VP4 (structural) and rotavirus enter toxin NSP4 (non-structural protein) has been developed. We validated the assay by comparing the results of single target PCR with dual-gene PCR products.|
|ISSN:||0975-0967 (Online); 0972-5849 (Print)|
|Appears in Collections:||IJBT Vol.19(1) [January 2020]|
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