NISCAIR Online Periodicals Repository

Research Journals >
Indian Journal of Experimental Biology (IJEB) >
IJEB Vol.45 [2007] >
IJEB Vol.45(09) [September 2007] >

Title: Factors affecting induction and development of in vitro rooting in apple rootstocks
Authors: Sharma, T
Modgil, M
Thakur, M
Keywords: Apple rootstocks
In vitro rooting
Issue Date: Sep-2007
Publisher: CSIR
Abstract: Shoots of apple rootstocks raised in vitro were transferred to various rooting media to study the effect of different factors on root initiation and development. Various concentrations of indole-3-butyric acid (IBA) initiated rooting but maximum rooting percentage was found with 2.0 and 2.5 mg l-1 of IBA in M7 and with 1.0 mg l-1 of IBA in MM106. The drawback was that the roots were thick, short and with profuse callus. The presence of activated charcoal (AC) in the rooting medium improved the rooting quality but reduced the rooting percentage in both the rootstocks. In high auxin dip of 70, 80 and 90 mg l-1 IBA for 2, 2 and 1 hr showed 75-85 per cent rooting in M7, but lacked reproducibility of the results. Whereas in MM106, 66 – 70 % rooting was achieved with 70 mg l-1 of IBA dip for 3 h. Root induction in shoots in IBA containing liquid medium (LM) in dark for few days and root elongation in IBA - free medium in light proved most effective. On the other hand, continuous light treatment showed reduced rooting. Reduction of MS salts and sucrose in root elongation medium showed decreased rooting. Plantlets from two - stage rooting procedure showed more rapid growth and satisfactory survival during hardening of plants and on transfer to field.
Page(s): 824-829
ISSN: 0975-1009 (Online); 0019-5189 (Print)
Source:IJEB Vol.45(09) [September 2007]

Files in This Item:

File Description SizeFormat
IJEB 45(9) 824-829.pdf169.53 kBAdobe PDFView/Open
 Current Page Visits: 868 
Recommend this item


Online Submission of Articles |  NISCAIR Website |  National Knowledge Resources Consortium |  Contact us |  Feedback

Disclaimer: NISCAIR assumes no responsibility for the statements and opinions advanced by contributors. The editorial staff in its work of examining papers received for publication is helped, in an honorary capacity, by many distinguished engineers and scientists.

CC License Except where otherwise noted, the Articles on this site are licensed under Creative Commons License: CC Attribution-Noncommercial-No Derivative Works 2.5 India

Copyright © 2015 The Council of Scientific and Industrial Research, New Delhi. All rights reserved.

Powered by DSpace Copyright © 2002-2007 MIT and Hewlett-Packard | Compliant to OAI-PMH V 2.0

Home Page Total Visits: 169786 since 01-Sep-2015  Last updated on 30-Jun-2016Webmaster: