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Title: Evaluation of DNA polymorphism in the Red Sea Epinephelus species using 12s rRNA and inter simple sequence repeats
Authors: Shaikh-Omar, A M
Saad, Y M
Al-Hasawi, Z M
Keywords: DNA polymorphism;Epinephelus;Genetics;ISSR;12s rRNA
Issue Date: Jul-2020
Publisher: NISCAIR-CSIR, India
Abstract: The true phylogenetic relation among Epinephelus species is under debate. The present investigation was designed for evaluation of DNA polymorphism in some Red sea Epinephelus species using 12s rRNA and Inter Simple Sequence repeats. DNA polymorphism values were detected and evaluated within all estimated fishes. Both applied techniques revealed that E. malabaricus is closely related to E. summana. The distance value between E. chlorostigma and E. areolatus is lower than the distance value between E. chlorostigma and E. radiatus. The Serranidae evolutionary variations were evaluated comparatively with other ray-finned fishes belonging to four fish genera (Carangidae, Labridae, Mugilidae and Cichlidae) based on 12s rRNA sequence variations (obtained from NCBI). The developed DNA markers were reliably branched in the evaluated fishes. More molecular investigations using more spatial and temporal fish samples should be carried out in the future for exploring the true Epinephelus species biodiversity in the Red sea.
Page(s): 1197-1205
ISSN: 0975-1033 (Online); 0379-5136 (Print)
Appears in Collections:IJMS Vol.49(07) [July 2020]

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