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Title: In vitro incompatibility study of Valsartan and Hydrochlorothiazide by spectroscopic and RP-HPLC Method
Authors: Kumar, M Manoj
Himaja, D
Sen, Sandip
Keywords: Valsartan;Hydrochlorothiazide;Spectroscopic method;RP-HPLC;In vitro drug interaction
Issue Date: Aug-2020
Publisher: NISCAIR-CSIR, India
Abstract: The present investigation is based on an in vitro incompatibility study between valsartan and hydrochlorothiazide by spectroscopic and reverse-phase high-pressure liquid chromatography (RP-HPLC) methods. The method has been developed and validated by ultraviolet (UV) spectroscopic method using methanol and water (1:4) as the solvent. The RP-HPLC study has been carried out using acetonitrile, methanol and 50 Mm phosphate buffer (20:50:30 %) adjusted to pH 3 with orthophosphoric acid. The methods show linearity at the concentration range of 1-5 μg/ml with correlation coefficient 0.9994 for valsartan and 0.9978 for hydrochlorothiazide in the concentration range of 1-5 μg/ml. Both the methods have shown a percentage relative standard deviation values less than 2. The in vitro incompatibility study has been carried out by UV-spectroscopic and RP-HPLC methods. The results of the study show that there is a change in the concentration level of both drugs in combination. The structure of the interacting compound has been determined by spectral analysis using IR, 1H NMR and LC-MS study. The spectral analysis confirms the formation of the new complex between valsartan and hydrochlorothiazide. The results obtained from the LC-MS study also correlate the reason behind an increase in the concentration of valsartan and decreases the concentration of hydrochlorothiazide during recovery study by spectroscopic and RP-HPLC method.
Page(s): 1120-1127
ISSN: 0975-0975(Online); 0376-4710(Print)
Appears in Collections:IJC-A Vol.59A(08) [August 2020]

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