Please use this identifier to cite or link to this item: http://nopr.niscair.res.in/handle/123456789/55052
Title: Interfacial and thermodynamic approach of surfactants with α-chymotrypsin and trypsin: A comparative study
Authors: Banjare, Ramesh Kumar
Banjare, Manoj Kumar
Ghosh, Kallol K
Mundeja, Prashant
Panda, Sandhyarani
Keywords: α-Chymotrypsin;Trypsin;Surfactant;Micellization Behavior;Conductivity;Surface tension
Issue Date: Aug-2020
Publisher: NISCAIR-CSIR, India
Abstract: This work deals with the interactions among cetyltrimethylammonium bromide (CTAB), sodium dodecyl sulfate (SDS) with α-chymotrypsin (α-CT) and trypsin in aqueous medium on pH 7.75 by conductivity and surface tension measurements. The critical micelle concentrations (CMC), surface parameters i.e., the maximum surface excess concentration (Γmax), minimum area per surfactant molecule (Amin), the surface pressure at CMC (πCMC) and thermodynamic parameters i.e., degree of ionization (α), Gibbs free energy of micellization (ΔG°m), the standard Gibbs energy of adsorption (ΔG°ads), the free energy at air-water interface (ΔGsmim) have been evaluated. The CMC has increased and surface tension of CMC (γCMC) values have decreased (at maximum μL of α-CT and trypsin), significantly in the presence of different μL of the added α-CT and trypsin. In this study, ΔG0ads value is established to be greater than ΔG0m, showing that adsorption is more favored in aqueous surfactants systems. Thermodynamic parameters show that enzyme-CTAB/SDS monomeric aggregation started to form micelles at a higher concentration of surfactant to compare with the CMC of pure CTAB/SDS micelles. It is significant that increasing the μL of α-CT and trypsin results in an increase in the spontaneity CMC on surfactants, α-CT and trypsin have more affinity for SDS compared to CTAB.
Page(s): 1128-1135
URI: http://nopr.niscair.res.in/handle/123456789/55052
ISSN: 0975-0975(Online); 0376-4710(Print)
Appears in Collections:IJC-A Vol.59A(08) [August 2020]

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