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Title: Molecular phylogeny and spectral characteristics of selected soil crust cyanobacteria from tea gardens of Darjeeling (India)
Authors: Kumar, Dhanesh
Bhakta, Sukumar
Adhikary, Siba Prasad
Keywords: Molecular phylogeny;Scytonemin;Biological soil crust;Himalaya;Darjeeling tea garden
Issue Date: Oct-2019
Publisher: NISCAIR-CSIR, India
Abstract: Soil crust cyanobacteria from three tea gardens of Mirik, Kurseong and Darjeeling located at different altitudes of Darjeeling district (West Bengal, India) in Himalayas, were studied. A total of 16 cyanobacteria were isolated in pure culture and identified. Major taxa in all soil crusts from three locations were under the genus Calothrix. 16S rRNA sequencing of three dominant species of Calothrix were performed and the sequences were submitted to National Center for Biotechnology Information (NCBI) GenBank and the accession numbers were KF499103, KF499104 and KF499105. Phylogenetic analysis with other similar organisms isolated across the globe showed their relatedness with Calothrix species. Absorbance spectra of 90% (v/v) methanolic extracts of the crusts and their major organisms in culture showed the presence of ultra-violet (UV) sunscreen pigment scytonemin and mycosporine like amino acids (MAAs).
Page(s): 330-336
ISSN: 0975-0967 (Online); 0972-5849 (Print)
Appears in Collections:IJBT Vol.18(4) [October 2019]

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