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Title: Green synthesis of silver nanoparticles from Citrus medica peels and determination of its antioxidant activity
Authors: Sati, S C
Bartwal, Ankit S
Agarwal, Alok Kumar
Keywords: Silver nanoparticles;Green synthesis;Citrus medica;Antioxidant activity
Issue Date: Mar-2020
Publisher: NISCAIR-CSIR, India
Abstract: Citrus medica commonly known as Nimboo, is an evergreen aromatic tree of Rutaceae family, with height of 4-5 m, commonly found in the Garhwal Himalayan region. Leaves are generally long, ovate –lanceolate, 10-12 cm long, Flowers auxiliary, 1.5-2 cm across, sweet scented. Fruits yellow, ovoid-subglobose, rough or warty, rind thick, pulp loose, aromatic, sweet & sour, juice sacs small, slender, seeds numerous, oval, commonly found in uncommon forest edges of outer Himalaya. Present study deals with the synthesis of Silver nanoparticles (AgNPs) from Citrus medica peels by green synthesis method, which does not involve any harmful chemicals. On mixing of AgNO3 solution and plant extract in respective ratios, the reduction of Ag+ ions take place due to presence of some water-soluble organic compounds. The reduction of Ag+ ion is observed with the help of UV-Vis absorption spectroscopy and the synthesized AgNPs were characterized by X-ray diffraction method followed by SEM, EDAX and HR-TEM studies. The results of UV-Vis absorption study show a strong absorption band of silver nanoparticles (AgNPs) at λmax 440 nm. The X-ray diffraction analysis confirmed that the synthesized AgNPs are cubic crystal solid. The SEM analysis of AgNPs shows average size of 1.35 μm, while the EDAX analysis confirmed the significant presence of silver with carbon, oxygen, chlorine and Iron as other contaminants. This analysis is followed by TEM which shows that the water-soluble AgNPs, have approximate size 53nm. The characterized AgNPs was also analysed for antioxidant activity. The IC50 value for BHT has been calculated as 6.4 mg/ml while for the AgNPs it is 19.3 mg/ml.
Page(s): 56-60
ISSN: 2581-8198 (Online)
Appears in Collections:AIR Vol.02(1) [March 2020]

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