Please use this identifier to cite or link to this item: http://nopr.niscair.res.in/handle/123456789/54356
Title: Adjuvant effect of Garlic extracts (Allium sativum L.) on the production of γ globulin in mice immunized with ovalbumin
Authors: Daniela, Estrada-Villa
Raúl, De Santiago-Castañón
Carolina, Barboza-Herrera
Flor-del-Carmen, Rivera-Barbosa
Sonia, López-García
Marcela, Torres-Hernández
Irais, Castillo-Maldonado
Luis-Benjamín, Serrano-Gallardo
Mario-Alberto, Rivera-Guillén
Dealmy, Delgadillo-Guzmán
David, Pedroza-Escobar
Keywords: Adjuvant;Allium sativum L;Garlic;Mouse;γ globulin
Issue Date: Apr-2020
Publisher: NISCAIR-CSIR, India
IPC Code: Int. Cl.20: A61K 39/39, A61K 36/8962, A61K 38/39
Abstract: The antigens used in vaccines are usually attenuated or inactivated microorganisms, toxoids or purified particles. The purified particles have a better biosecurity but their capacity to generate an immune response is low, therefore vaccines include adjuvants that seek to improve immunogenicity. Unfortunately, adjuvants have side effects so only aluminum salts are currently used as adjuvants. So that this work evaluated an adjuvant of garlic extracts, a plant with immunomodulatory properties, in mice immunized with ovalbumin. To formulate the adjuvant, biotoxicity and cytotoxicity assays with a model of Artemia salina and haemolytic activity were considered respectively. A qualitative phytochemical analysis and quantification of phenolic compounds were carried out and in the immunization scheme 100 μg of antigen with adjuvant were administered at day 1, 50 and 100 μg of antigen on days 14 and 28 respectively. The sacrifice of the animals was done on day 30. Leukocytes and γ globulins were quantified at the beginning and at the end of the experiment. A lethal dose 50% of 1430 μg/mL was calculated for the Garlic extracts, a haemolytic activity of 2.66% and 7.53% was observed (p<0.05) for the concentrations of 10 and 100 μg/mL. And it was only possible to identify the presence of tannins in the aqueous extract of Garlic. With the results obtained, significant differences were observed in leukocyte counts and concentration of γ globulins at the end of the immunization scheme (p<0.05). Concluding that the results with the adjuvant of Garlic at 10 μg/mL concentration were comparable to those found with the adjuvant of aluminum salts.
Page(s): 299-306
URI: http://nopr.niscair.res.in/handle/123456789/54356
ISSN: 0975-1068 (Online); 0972-5938 (Print)
Appears in Collections:IJTK Vol.19(2) [April 2020]

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