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Title: Carborundum-dependent entrance of<i> Eco</i>RI restriction enzyme into plant cells and specific cleavage of genomic DNA
Authors: Gholizadeh, Ashraf
Kohnehrouz, B Baghban
Keywords: Cleavage
Plant cell
Restriction enzyme
Issue Date: Aug-2009
Publisher: CSIR
Abstract: In a basic research to determine the morpho-molecular interactions of plant tissues with <i>Eco</i>RI DNA restriction enzyme, it was demonstrated that this protein is capable of entering the sunflower and maize leaf cells using a plant tissue-abrading material and cleaving the genomic DNA at specific sites. This was inferred from the analysis of morphological patterns of <i>Eco</i>RI-treated leaf areas as well as using some molecular tests, including the cleavage pattern analysis of genomic DNA isolated from treated locations followed by ligation of cleaved fragments into <i>Eco</i>RI site of a DNA cloning vector system. The overall results indicated that the specific restriction of genomic DNA may happen following the entrance of <i>Eco</i>RI protein most likely into the nucleus of plant cells.
Description: 684-689
ISSN: 0975-1009 (Online); 0019-5189 (Print)
Appears in Collections:IJEB Vol.47(08) [August 2009]

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