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Title: A novel compound β-sitosterol-3-O-β-D-glucoside isolated from Azadirachta indica effectively induces apoptosis in leukemic cells by targeting G0/G1 populations
Authors: Sarkar, Swadesh
Pal, Amrita
Chouni, Anirban
Paul, Santanu
Keywords: Anti-proliferative;Cytotoxicity;Leukemia;Neem;β-sitosterol-3-O-β-D-glucoside
Issue Date: Feb-2020
Publisher: NISCAIR-CSIR, India
Abstract: Azadirachta indica, popularly known as ‘Neem’, is an important plant in the Ayurveda system of medicine. It is known to prevent about at least 40 types of diseases in home-practice. A previous report showed that the methanolic extract of this plant can effectively control proliferation of leukemia cells. Here, we explored the eEthanolic extract dried neem leaves for a new molecule with potent anti-leukemic property.Four biomolecules were isolated viz. rutin, isoquercetin, quercetrin and β-sitosterol-3-O-β-D-glucoside from the ethanolic extract by repeated column chromatography and HPLC.Quercetrin structures of the isolated molecules were confirmed by Mass, 1HNMR, 13C NMR spectra analysis. MTT assay revealed that β-sitosterol-3-O-β-D-glucoside effectively reduced the proliferation of MOLT 4 leukemic cells in a dose and time dependent manner. DAPI staining with confocal microscopy indicated that β-sitosterol-3-O-β-D-glucoside efficiently induced nuclear DNA fragmentation in MOLT 4 cells. Finally, flow cytometry after PI staining showed that the compound has potential to check the cell cycle progression at sub G1 phase. With the available results, it can be concluded that β-sitosterol-3-O-β-D-glucoside has potential to be good therapeutic drug in leukemia treatment.
Page(s): 27-32
ISSN: 0975-0959 (Online); 0301-1208 (Print)
Appears in Collections:IJBB Vol.57(1) [February 2020]

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