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JSIR Vol.64 [2005] >
JSIR Vol.64(12) [December 2005] >
| Title: | An extracellular protease with depilation activity from Streptomyces nogalator |
| Authors: | Mitra, P
Chakrabartty, P K |
| Keywords: | Depilation
Enzyme purification
Proteolytic enzyme
Streptomyces nogalator |
| Issue Date: | Dec-2005 |
| Publisher: | CSIR |
| IPC Code: | C12N11/02 |
| Abstract: | An extracellular protease-producing actinomycete, Streptomyces nogalator strain Ac 80, was isolated from soil. Mutants of the strain designated as Mt C producing protease of higher (3.0 mg tyrosine liberated/min/mg protein) and Mt L of lower (0.9 mg tyrosine liberated/min/mg protein) specific activity as compared to that of the wild type (1.8 mg tyrosine liberated/min/mg protein) were selected. Proteolytic activity of the wild type and the mutants correlated with their depilation capacities. Proteolytic enzyme produced by Mt C in solid state fermentation was purified to homogeneity, and partially characterized (molecular mass, 66 kDa by SDS- PAGE). It was found to be efficient in depilation of goatskin. |
| Page(s): | 978-983 |
| ISSN: | 0975-1084 (Online); 0022-4456 (Print) |
| Source: | JSIR Vol.64(12) [December 2005]
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