NISCAIR Online Periodicals Repository

Research Journals >
Indian Journal of Experimental Biology (IJEB) >
IJEB Vol.45 [2007] >
IJEB Vol.45(11) [November 2007] >

Title: Purification of elastase-like chymotrypsin from cardamom shoot and Capsule borer
Authors: Josephrajkumar, A
Chakrabarty, R
Thomas, G
Keywords: Aprotinin
Conogethes punctiferalis
Issue Date: Nov-2007
Publisher: CSIR
Abstract: An elastase-like chymotrypsin was purified by aprotinin-agarose affinity chromatography from the midgut extract of cardamom shoot and capsule borer, Conogethes punctiferalis. The purified enzyme had a Vmax of 687.6 ± 22.1 nmole pNA released/min/mg protein, Km of 0.168 ± 0.012 mM with SAAPLpNA as substrate and gave a single band on SDS-PAGE with a molecular mass of 72.1 kDa. Casein zymogram revealed one clear zone of proteolytic activity, which corresponded to the band obtained with SDS-PAGE indicating that this could be a single-polypeptide enzyme.
Page(s): 998-1002
ISSN: 0975-1009 (Online); 0019-5189 (Print)
Source:IJEB Vol.45(11) [November 2007]

Files in This Item:

File Description SizeFormat
IJEB 45(11) 998-1002.pdf206.56 kBAdobe PDFView/Open
 Current Page Visits: 826 
Recommend this item


Online Submission of Articles |  NISCAIR Website |  National Knowledge Resources Consortium |  Contact us |  Feedback

Disclaimer: NISCAIR assumes no responsibility for the statements and opinions advanced by contributors. The editorial staff in its work of examining papers received for publication is helped, in an honorary capacity, by many distinguished engineers and scientists.

CC License Except where otherwise noted, the Articles on this site are licensed under Creative Commons License: CC Attribution-Noncommercial-No Derivative Works 2.5 India

Copyright © 2015 The Council of Scientific and Industrial Research, New Delhi. All rights reserved.

Powered by DSpace Copyright © 2002-2007 MIT and Hewlett-Packard | Compliant to OAI-PMH V 2.0

Home Page Total Visits: 171346 since 01-Sep-2015  Last updated on 30-Jun-2016Webmaster: