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Research Journals >
Indian Journal of Experimental Biology (IJEB) >
IJEB Vol.45 [2007] >
IJEB Vol.45(11) [November 2007] >
| Title: | Purification of elastase-like chymotrypsin from cardamom shoot and Capsule borer |
| Authors: | Josephrajkumar, A
Chakrabarty, R
Thomas, G |
| Keywords: | Aprotinin
Cardamom
Chymotrypsin
Conogethes punctiferalis
Trypsin |
| Issue Date: | Nov-2007 |
| Publisher: | CSIR |
| Abstract: | An elastase-like chymotrypsin was purified by aprotinin-agarose affinity chromatography from the midgut extract of cardamom shoot and capsule borer, Conogethes punctiferalis. The purified enzyme had a Vmax of 687.6 ± 22.1 nmole pNA released/min/mg protein, Km of 0.168 ± 0.012 mM with SAAPLpNA as substrate and gave a single band on SDS-PAGE with a molecular mass of 72.1 kDa. Casein zymogram revealed one clear zone of proteolytic activity, which corresponded to the band obtained with SDS-PAGE indicating that this could be a single-polypeptide enzyme. |
| Page(s): | 998-1002 |
| ISSN: | 0975-1009 (Online); 0019-5189 (Print) |
| Source: | IJEB Vol.45(11) [November 2007]
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