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IJEB Vol.45(11) [November 2007] >


Title: Purification of elastase-like chymotrypsin from cardamom shoot and Capsule borer
Authors: Josephrajkumar, A
Chakrabarty, R
Thomas, G
Keywords: Aprotinin
Cardamom
Chymotrypsin
Conogethes punctiferalis
Trypsin
Issue Date: Nov-2007
Publisher: CSIR
Abstract: An elastase-like chymotrypsin was purified by aprotinin-agarose affinity chromatography from the midgut extract of cardamom shoot and capsule borer, Conogethes punctiferalis. The purified enzyme had a Vmax of 687.6 ± 22.1 nmole pNA released/min/mg protein, Km of 0.168 ± 0.012 mM with SAAPLpNA as substrate and gave a single band on SDS-PAGE with a molecular mass of 72.1 kDa. Casein zymogram revealed one clear zone of proteolytic activity, which corresponded to the band obtained with SDS-PAGE indicating that this could be a single-polypeptide enzyme.
Page(s): 998-1002
ISSN: 0975-1009 (Online); 0019-5189 (Print)
Source:IJEB Vol.45(11) [November 2007]

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