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Title: Isolation of <i style="">Mycobacterium tuberculosis </i>protein antigens ES-31, ES-43 and EST-6 of diagnostic interest from Tubercle Bacilli by affinity chromatography
Authors: Upadhye, Vijay
Saha-Roy, Santa
Shende, Niraj
Kumar, Satish
Harinath, B C
Keywords: Affinity chromatography
DSS antigens
<i style="">Mycobacterium tuberculosis</i>
Pulmonary tuberculosis
Issue Date: Jul-2007
Publisher: CSIR
Abstract: Immunodiagnostically useful <i style="">M. tuberculosis </i>H<sub>37</sub>Ra protein antigens ES-31, ES-43 and EST-6 were isolated from detergent soluble sonicate (DSS) antigen using monospecific antibodies by affinity chromatography and compared with similar antigens isolated from <i style="">M. tuberculosis </i>culture filtrate for seroreactivity in tuberculosis sera by Indirect Enzyme Linked Immunosorbent Assay. Recovery of affinity purified ES-31, ES-43 and EST-6 antigen from DSS antigen was approximately 3, 3.5 and 4% respectively, compared to 10, 9 and 6.3% from culture filtrate. Affinity purified ES-31, ES-43 and EST-6 antigens from both culture filtrate as well as DSS antigen showed similar seroreactivity with overall sensitivity 85, 80 and 75% respectively and specificity of 85% at optimum concentration of 50 pg protein of each antigen. The results suggest that DSS antigen may be a promising antigen source for isolating antigens of diagnostic interest obviating the need for cumbersome, time-consuming culture techniques of mycobacteria.
Description: 599-602
ISSN: 0975-1009 (Online); 0019-5189 (Print)
Appears in Collections:IJEB Vol.45(07) [July 2007]

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