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Title: Evaluation of <i style="">in vitro</i> antioxidant activity of Indian bay leaf, <i style="">Cinnamomum tamala </i>(Buch. -Ham.) T. Nees & Eberm<i style=""> </i>using rat brain synaptosomes as model system
Authors: Devi, S Lakshmi
Kannappan, S
Anuradha, C V
Keywords: Antiradical property
Indian bay leaf
Lipid peroxidation
Oxidative stress
Issue Date: Sep-2007
Publisher: CSIR
Abstract: The study investigated the perturbation of oxidant-antioxidant balance in brain synaptosomes of diabetic rats and determined the antioxidant and free radical-scavenging property of the Indian bay leaf. Brain synaptosomes were isolated from control and streptozotocin-induced diabetic animals and oxidative stress parameters were assayed. A methanolic extract of bay leaf (BLE) was tested for the polyphenolic content and antioxidant activity by <i style="">in vitro</i> assays. A significant increase in the levels of lipids and lipid peroxidation products and a decline in antioxidant potential were observed in diabetic rat brain synaptosomes. The total polyphenolic content of BLE was found to be 6.7mg gallic acid equivalents (GAE)/100g. BLE displayed scavenging activity against superoxide and hydroxyl radicals in a concentration-dependent manner. Further, BLE showed inhibition of Fe<sup>2+</sup>-ascorbate induced lipid peroxidation in both control and diabetic rat brain synaptosomes. Maximum inhibition of lipid peroxidation, radical scavenging action and reducing power of BLE were observed at a concentration of 220 <img src='/image/spc_char/micro.gif'> g GAE. These effects of BLE <i style="">in vitro</i> were comparable with that of butylated hydroxyl toluene (BHT), a synthetic antioxidant. It can be concluded that synaptosomes from diabetic rats are susceptible to oxidative damage and the positive effects of bay leaf <i style="">in vitro</i>, could be attributed to the presence of antioxidant phytochemicals.
Description: 778-784
ISSN: 0975-1009 (Online); 0019-5189 (Print)
Appears in Collections:IJEB Vol.45(09) [September 2007]

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