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IJEB Vol.45(08) [August 2007] >


Title: Expression of mRNAs encoding tumor necrosis factor- and its receptor–I in buffalo ovary
Authors: Madhusudan, G P
Dev, R
Sharma, M K
Singh, Dheer
Keywords: Expression
mRNA
TNF-
TNFRI
RT-PCR
Ovary
Buffalo
Issue Date: Aug-2007
Publisher: CSIR
Abstract: The tumor necrosis factor- (TNF- ) plays an important role in ovarian follicular development and ovulation process and acts through its receptor (TNFRI). The present investigation describes the expression of mRNAs encoding TNF- and TNFRI in relation to glyceraldehyde-3-phosphate dehydrogenase (G3PDH) and β-actin as control genes, using RT-PCR, in granulosa cells, intact follicles and luteal tissues from buffalo ovary. There was significant higher expression of mRNAs encoding TNF- in granulosa cells from medium follicles and TNFRI expression increased with increase in size of follicles. Post-ovulatory structures (corpus luteum and corpus albicans) exhibited significantly higher expression of TNFRI mRNAs as compared to that obtained in intact follicles suggesting its immediate and critical role just after ovulation, for mediating TNF- action on these tissues. Though the expression of TNF- mRNA was stimulated by treatment of granulosa cells with FSH during culture, the expression of TNFRI mRNA did not change. The FSH alongwith IGF-I did not exert any effect. These results suggested an important role of TNF- and its receptor in buffalo ovarian functions.
Page(s): 669-675
ISSN: 0975-1009 (Online); 0019-5189 (Print)
Source:IJEB Vol.45(08) [August 2007]

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